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MetabolomicsMass spectrometry imaging as an emerging tool for studying metabolism in human brain organoids

Human brain organoids are emerging models to study human brain development and pathology as they recapitulate the development and characteristics of major neural cell types, and enable manipulation through an in vitro system. Over the past decade, with the advent of spatial technologies,...

2023-05-23 | MTBLS7565 | MetaboLights
ProteomicsModelling Synaptic Maturation from Growth Cone to Synapse in Human Organoids
Human neural organoids (NOs) provide a powerful platform for investigating synaptic development and dysfunction during early neurodevelopment. However, methodologies for isolating functional synaptic structures from these models remain limited. Here, we present a differential centrifugation protocol...
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2026-05-25 | PXD072589 | Pride
Synapse Synaptosomes Guided dorsal forebrain organoid Stimulation Nerve terminals Dynamin. Subcellular enrichment Proteomics Neural organoid Brain organoid Gap43 Phosphorylation Air-liquid interface cerebral organoid Growth cone Differential centrifugation 
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