Sort   by:  
 Page size 
TranscriptomicsPrp8 Retinitis Pigmentosa Mutants Cause Defects in the Transition between the Catalytic Steps of Splicing
The essential process of pre-mRNA splicing must occur with high fidelity and efficiency for proper gene expression. The spliceosome employs DExD/H box helicases to promote on-pathway interactions while simultaneously minimizing errors. Prp8 and Snu114, an EF2-like GTPase, regulate the activity of th...
ORGANISM(S): Saccharomyces cerevisiae 
2016-07-21 | E-GEOD-75081 | biostudies-arrayexpress
TranscriptomicsA single SR-like protein, Npl3, promotes pre-mRNA splicing in budding yeast
Mammalian SR proteins are a family of reversibly phosphorylated RNA binding proteins primarily studied for their roles in alternative splicing. While budding yeast lack alternative splicing, they do have three SR-like proteins: Npl3, Gbp2, and Hrb1. However, these have been primarily studied for the...
ORGANISM(S): Saccharomyces cerevisiae 
2008-12-30 | E-GEOD-11491 | biostudies-arrayexpress
TranscriptomicsStructural evidence for consecutive Hel308-like modules in the spliceosomal ATPase Brr2
Brr2 is a DExD/H-box helicase responsible for U4/U6 unwinding, a critical step in spliceosomal activation. Brr2 contains an N-terminal domain and two tandem sets of a helicase-like domain followed by a Sec63 domain with unknown function. We determined the crystal structure of the second Sec63 doma...
ORGANISM(S): Saccharomyces cerevisiae 
2010-05-18 | E-GEOD-16135 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of wild type and dbr1-delta strains of Candida albicans grown under standard or various mixed conditions
Probes flanking all predicted Candida albicans ORFs are used to detect intron sequences that accumulate in a debranchase (DBR1) mutant. We synthesized cDNA from RNA from both wild-type and dbr1-delta mutant strains grown under standard (YEPD) or various mixed conditions, labeled the two populations...
ORGANISM(S): Candida albicans 
2007-02-22 | E-MEXP-1003 | biostudies-arrayexpress
TranscriptomicsA Genetic Interaction Map of RNA Processing Factors Reveals Links Between mRNA Processing Complexes
All classes of RNA undergo processing. The two largest RNA processing complexes are those involved in rRNA biogenesis and mRNA splicing. Because of their complexity and essential roles, it has been difficult to dissect the functional interactions within these machines as well as to identify possible...
ORGANISM(S): Saccharomyces cerevisiae 
2008-12-30 | E-GEOD-11634 | biostudies-arrayexpress
TranscriptomicsDiverse Environmental Stresses Elicit Distinct Responses at the Level of Pre-mRNA Processing in Yeast.
Gene expression in Eukaryotic cells is profoundly shaped by the post-transcriptional processing of mRNAs, including the splicing of introns in the nucleus and both nuclear and cytoplasmic degradation pathways. Here we report the use of a splicing isoform specific microarray platform to investigate ...
ORGANISM(S): Saccharomyces cerevisiae 
2011-07-01 | E-GEOD-28919 | biostudies-arrayexpress
TranscriptomicsLoss of the yeast SR protein Npl3 alters gene expression due to transcription readthrough
Yeast Npl3 is a highly abundant RNA binding protein, related to metazoan SR proteins, with reported functions including transcription elongation, splicing and RNA 3’ end processing. To identify direct targets and functions for Npl3, we used UV crosslinking and analysis of cDNA (CRAC) to map precise ...
ORGANISM(S): Saccharomyces cerevisiae S288c 
2015-12-02 | E-MTAB-3642 | biostudies-arrayexpress
TranscriptomicsSchizosaccharomyces pombe splicing microarray
Analysis of splicing defects in Schizosaccharomyces pombe upon chemical genetic inhibition of splicing kinases dsk1, lkh1, and prp4, as well as alanine-mutation of phosphorylated residues in the splicing factors bpb1, prp2, rsd1, srp1, srp2, usp101, usp103, sum3, prp22, cdc5, and cwf22. This study s...
ORGANISM(S): Schizosaccharomyces pombe 
2015-07-13 | E-GEOD-62752 | biostudies-arrayexpress
TranscriptomicsKaplan2013: High-Resolution, Quantitative Genetic Analysis of RNA Polymerase II
Dynamic control of gene expression is crucial for most aspects of cell physiology and at its core is RNA polymerase II (RNAPII). Using 53 RNAPII point mutants, we generated a point mutant epistatic miniarray profile (pE-MAP) comprising ~60,000 quantitative genetic interactions in Saccharomyces cere...
ORGANISM(S): Saccharomyces cerevisiae 
2013-08-06 | E-GEOD-47429 | biostudies-arrayexpress
Sort   by:  
 Page size