Kaplan2013: High-Resolution, Quantitative Genetic Analysis of RNA Polymerase II

ABSTRACT: Dynamic control of gene expression is crucial for most aspects of cell physiology and at its core is RNA polymerase II (RNAPII). Using 53 RNAPII point mutants, we generated a point mutant epistatic miniarray profile (pE-MAP) comprising ~60,000 quantitative genetic interactions in Saccharomyces cerevisiae. This enabled functional assignment of RNAPII sub-domains, including connections to protein complexes. Using splicing microarrays and point mutants altering elongation rates in vitro, we found an inverse relationship between RNAPII speed and in vivo splicing efficiency. Furthermore, the pE-MAP classified groups of fast and slow mutants that favor upstream and downstream start site selection, respectively. Finally, the pE-MAP identified Sub1 as a positive transcription factor regulatin

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Marian Groot Koerkamp 

PROVIDER: E-GEOD-47429 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress