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Proteomicsyeast
yeast
ORGANISM(S): Saccharomyces Cerevisiae 
2003-12-31 | PAe000145 | PeptideAtlas
ProteomicsMitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria
Cells harbor two systems for fatty acid synthesis, one in the cytoplasm (catalyzed by fatty acid synthase, FASN) and one in the mitochondria (mtFAS). In contrast to FASN, mtFAS is poorly characterized, especially in higher eukaryotes, with the major product(s), metabolic roles, and cellular function...
ORGANISM(S): Homo sapiens (Human) 
2020-09-02 | PXD021034 | Pride
Metabolism Mitochondria Electron transport chain Fatty acid synthesis Oxphos Respiration Reductive carboxylation Lipoylation tca cycle Muscle 
Proteomics,MultiomicsHs_NSCLC_Phospho
This is a large-scale survey of tyrosine kinase activity in lung cancer. Tyrosine kinase signaling across 41 non-small cell lung cancer (NSCLC) cell lines and over 150 NSCLC tumors are characterized Using a phosphoproteomic approach.
ORGANISM(S): Homo Sapiens (human) 
2007-12-31 | PAe005321 | PeptideAtlas
ProteomicsProteome-wide evaluation of two common protein quantification methods
Proteomics experiments commonly aim to estimate and detect differential abundance across all expressed proteins. Within this experimental design, some of the most challenging measurements are small fold changes for lower abundance proteins. While bottom-up proteomics methods are approaching comprehe...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Saccharomyces Cerevisiae (ncbitaxon:4932) 
2019-02-15 | MSV000083453 | MassIVE
ProteomicsA mass-tolerant database search identifies a large proportion of unassigned spectra in shotgun proteomics as modified peptides
Less than half of all MS/MS spectra acquired in shotgun proteomics typically result in a confident peptide match. Here we present an ultra-tolerant Sequest database search that allowed peptide matching even with modifications of unknown masses up to ±500 Da. From an HEK293 cell proteome-wide datas...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-06-19 | MSV000079841 | MassIVE
TranscriptomicsAnalysis of HeLa cells after transfection with miR-1, miR-124 and miR-181.
Analysis of HeLa cells at 24 hours after transfection with wild type miR-1, miR-124, miR-181 versus control transfected HeLa cells. Results were compared to protein down-regulation at 48 hours measured by SILAC-MS. Analysis of HeLa cells at 24 hours after transfection with wild type miR-1, miR-124,...
ORGANISM(S): Homo sapiens 
2010-05-25 | E-GEOD-11968 | biostudies-arrayexpress
ProteomicsNon-coding divergence of the human X and Y chromosomes drove male-biased expression of dosage-sensitive regulators
Little is known about how Y-chromosome gene expression directly contributes to differences between XX (female) and XY (male) individuals in non-reproductive tissues. It is often said that Y-chromosome genes show low expression outside the testis. Here, we analyzed quantitative profiles of Y-chromoso...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD017055 | Pride
Tandem mass tags Proteomics Orbitrap lumos Lc-ms/ms 
ProteomicsSample multiplexing-based targeted pathway proteomics with real-time analytics reveals the impact of genetic variation on protein expression
Targeted proteomics enables hypothesis-driven research by measuring the cellular expression of protein cohorts related by function, disease, or class after perturbation. Here, we present a pathway-centric approach and an assay builder resource for targeting entire pathways of proteins selected from ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090) 
2022-08-11 | MSV000090110 | MassIVE
ProteomicsProteome-wide evaluation of two common protein quantification methods
Proteomics experiments commonly aim to estimate and detect differential abundance across all expressed proteins. Within this experimental design, some of the most challenging measurements are small fold changes for lower abundance proteins. While bottom-up proteomics methods are approaching comprehe...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2018-04-05 | PXD007683 | Pride
Tmt Lfq Maxquant 
ProteomicsEvaluating False Transfer Rates from the Match-Between-Runs algorithm with a two-proteome model
Stochasticity between independent LC-MS/MS runs is a challenging problem in the field of proteomics resulting in significant missing values (i.e., abundance measurements) among observed peptides. To address this issue, several approaches have been developed including computational methods such as Ma...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2019-09-25 | PXD014415 | Pride
False transfer rate Match-between-runs Label-free quantitation Two-proteome mixture 
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