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no omics definedTranscription profiling of Listeria monocytogenes EGD-e after heat-shock for 3, 10, 20, and 40 min
L. monocytogenes EGD-e (Glaser et al., 2001) was grown in Brain Hearth Infusion Broth (Difco) with shaking (200 rpm, New Brunswick C24KC) using 10 ml culture medium in 100 ml conical flasks. An exponentially growing culture was used to inoculate 100 ml of fresh pre-warmed BHI broth in a 500 ml flask...
ORGANISM(S): Listeria monocytogenes 
2007-10-09 | E-MEXP-1118 | biostudies-arrayexpress
TranscriptomicsmicroRNA response of Galleria mellonella to Listeria monocytogenes infection: a powerful method to predict protein function in mammals exemplified by the autophagy adaptor optineurin
The Galleria mellonella larvae were infected with Listeria monocytogenes and on the 5th of post infection RNA is isolated from infected and non-infected control larvae. RNA samples were processed for miRNA profile in response to L. monocytogenes infection in Galleria mellonella larvae.
ORGANISM(S): Galleria mellonella 
2017-10-30 | E-MTAB-4024 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Listeria monocytogenes EGD-e versus the over-expressing strain (EGD-e+pPXrnhB
Bacteria were grown in BHI until OD600 1.0 (late exponential phase) and RNA stabilized using the RNAprotectTM bacteria reagent (QIAGEN) according to the manufacturer recommendations. For each strain, RNA extraction has been carried out on three independent biological samples. Briefly, bacterial tota...
ORGANISM(S): Listeria monocytogenes 
2008-06-29 | E-MEXP-1162 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of wild type Listera monocytogenes EGD-e and an isogenic deltahlydeltaplcA mutant strain 1 hour or 4 hours post infection into human intestinal epithelial cells
We infected wild type L. monocytogenes EGD-e (1) and its isogenic deltahlydeltaplcA (2) (lacking the ability to breach the vacuolar compartment of host cells following uptake) mutant strain to human intestinal epithelial cell line (Caco-2) with an MOI of 100 and 500 respectively. Bacterial total RNA...
ORGANISM(S): Listeria monocytogenes 
2008-06-18 | E-MEXP-1144 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Listeria monocytogenes intracellularly replicating in mouse macrophages activated by IFN-gamma
The present study show the impact of macrophages activation by IFN-Gamma on the gene regulation in intracellularly replicating L. monocytogenes and provide an overview about additionally adaption mechanisms of L. monocytogenes to immune response.
ORGANISM(S): Listeria monocytogenes 
2010-03-11 | E-TABM-663 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of wild type and sigma B mutant Liseteria monocytogenes to characterise the EGD-e sigma B regulon
Briefly, over night cultures of L. monocytogenes EGD-e cells (wild-type and sigma B mutant) were grown in brain heart infusion (BHI) broth (Difco). Bacteria were diluted 1:50 in 20 ml fresh BHI and incubated using a 100 ml Erlenmeyer flask at 37 °C with shaking (180 rpm, Unitron [Infors]). Bacterial...
ORGANISM(S): Listeria monocytogenes 
2008-07-02 | E-MEXP-1170 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Listeria monocytogenes after infection into murine macrophages
P388D1 murine macrophages were cultured in 85 mm tissue culture plates to about semi confluency. L. monocytogenes serotypes (1/2a EGD-e, 4a L99, 4b CLIP80459 and 4b F2365) were infected to the P388D1 cell monolayer at a MOI of 100 per eukaryotic cell. Infection was carried for 45 min and followed by...
ORGANISM(S): Listeria monocytogenes 
2010-11-25 | E-MEXP-1947 | biostudies-arrayexpress
MetabolomicsGlycerophospholipid remodeling is critical for orthoflavivirus infection

Flavivirus infection is tightly connected to host lipid metabolism. Here, we performed shotgun lipidomics of cells infected with neurotropic Zika, West Nile and tick-borne encephalitis viruses, as well as dengue and yellow fever virus. Early in infection specific lipids accumulated, e.g., neutral...

2025-02-24 | MTBLS11030 | MetaboLights
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