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The heated aldehyde-MPs solution was washed twice with NH₄HCO₃ (200 µL of 50 mM) buffer solution and then centrifuged (12,000 rpm, 5 min). Samples were alkylated with 5 μL of iodoacetamide for 1 h, subsequently undergoing tryptic digestion at 37 °C for 16 h (enzyme-to-protein ratio 1:50, w/w). The d...
ORGANISM(S): Bos Taurus 
2026-05-29 | PXD079095 |
Methods: RNA-seq libraries were prepared using the Illumina TruSeq RNA kit and the TrueSeq method was employed for mRNA enrichment. The libraries were quantified and samples were multiplexed in each lane of the flowcell. Cluster generation was performed and then sequenced on the Illumina HiSeq1000 s...
ORGANISM(S): Homo sapiens 
Anti-Flag antibody was added to the lysate of HUVECs transfected with Flag-FGF13 for co-immunoprecipitation, and IgG was used as a negative control. LC-MS/MS analysis was carried out by PTM Bio Co., Ltd (Zhejiang, China). The substrate proteins that may bind to FGF13 were screened out according to t...
ORGANISM(S): Homo Sapiens 
2026-06-16 | PXD079802 |
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