Sort   by:  
 Page size 
TranscriptomicsDNA replication profiling by BrdU Immunoprecipitation of S.cerevisiae wild-type versus H3K37R mutant
Wild-type and isogenic H3K37R yeast cultures were sinchronized in G1 with alpha factor. Cell were released into the cycle in the presence of 200mM Hydroxyurea and 0.5mg/ml BrdU. After 1h 15 minutes at 30C, replication was stopped by addition of NaN3 and cells were processed for DNA immunoprecipitati...
ORGANISM(S): Saccharomyces cerevisiae 
2021-04-14 | E-MTAB-9097 | biostudies-arrayexpress
GenomicsDNA replication profiling by BrdU Immunoprecipitation of S.cerevisiae wild-type versus H3K37R mutant under over-expression of MCM activators.
Wild-type and isogenic H3K37R yeast cultures were grown in medium containing 2% raffinose as carbone source. Cells were synchronized in G1 with alpha factor. To half culture (100ml), glucose (2% final concentration) and 1.3ml BrdU (50mg/ml stock) and 1.3ml BrdU (50mg/ml stock) was added. To the othe...
ORGANISM(S): Saccharomyces cerevisiae 
2021-04-30 | E-MTAB-9999 | biostudies-arrayexpress
TranscriptomicsRNA-seq expression profiling of OCI-AML2 and OCI-AML3 cells upon CRISPR/Cas9 –mediated disruption of DDX3X RNA binding domain
Human acute myeloid leukemia cell lines OCI-AML2 and OCI-AML3 were used in a CRISPR/Cas9-mediated approach to specifically target DDX3X’s gene sequences encoding the RNA binding domain of the helicase. DDX3X RNA binding domain is bipartite in the two halves of the helicase core. sgRNAs were designed...
ORGANISM(S): Homo sapiens 
2019-11-23 | E-MTAB-6241 | biostudies-arrayexpress
UnknownTIGERi: modeling and visualizing the responses to perturbation of a transcription factor network.
Not available
S-EPMC5471961 | biostudies-literature
TranscriptomicsExpression profiling of DDX3X and DDX54 knockdown in MCF7 cells
We performed RNA-seq to observe the gene expression changes in cells following siRNA-mediated knockdown of DDX3X and DDX54 RNA helicases in human breast cancer MCF7 cells. Two siRNAs were used to target each RNA helicase and scramble siRNA-treated MCF7 cells were used as controls.
ORGANISM(S): Homo sapiens 
2018-11-21 | E-MTAB-6235 | biostudies-arrayexpress
GenomicsChIPseq of DDX3X, DDX54, ERalpha and H3K4me3 in human breast cancer MCF7 cells
We aimed to investigate the chromatin binding activity of DDX3X and DDX54 RNA helicases in human ER -dependent breast cancer MCF7 cells. We run a parallel chromatin binding profiling of ER ChIP-seq. H3K4me3 profiling was used as a quality control of the ChIP-seq procedure.
ORGANISM(S): Homo sapiens 
2019-11-14 | E-MTAB-6211 | biostudies-arrayexpress
TranscriptomicsPKC1 cell cycle study
Transcriptional profiling of a Saccharomyces cerevisiae protein kinase Pkc1p temperature sensitive mutant on cells synchronised in S phase and then released. The Mcm1p-Fkh2p-Ndd1p complex in Saccharomyces cerevisiae controls the cyclical expression of the CLB2 cluster of genes at the G2/M phase tran...
ORGANISM(S): Saccharomyces cerevisiae 
2013-03-14 | E-MEXP-3667 | biostudies-arrayexpress
Sort   by:  
 Page size