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ProteomicsIdentifying Drug Targets with Thermal Proteome Profiling using IBT-16plex
Application of 16-plex iBT to drug target identification
ORGANISM(S): Homo Sapiens 
2023-03-30 | PXD041275 |
ProteomicsEvaluation of the Effect of Trypsin Digestion Buffers on Artificial Deamidation
This work comprehensively compared 50 mM of ammonium acetate (pH 6), Tris-HCl (pH 8), ABC and TEAB as in-solution trypsin digestion buffers. Both iTRAQ quantification and label-free results indicate that ammonium acetate (pH 6) is more suitable than other buffers for studying endogenous deamidation ...
ORGANISM(S): Rattus norvegicus (Rat) 
2022-02-28 | PXD001650 | Pride
Rat Kidney Artificial deamidation 
ProteomicsMulti-parameter optimization of two common proteomics quantification methods for quantifying low-abundance proteins
To improve the sensitivity and accuracy of label-free quantification and tandem mass tags (TMT) labeling in quantifying low-abundance proteins, multi-parameter optimization was carried out using a complex 2-proteome artificial sample mixture for a series of steps from sample preparation to data anal...
ORGANISM(S): Homo Sapiens Mus Musculus 
2018-11-02 | PXD011574 |
ProteomicsMouse Stromal Cells Confound Proteomic Characterization and Quantification of Xenograft Models
Accurate Protein Characterization and Quantification of Xenograft Tumors Requires Separating Human and Mouse Cells prior to Proteomics Profiling
ORGANISM(S): Homo Sapiens Mus Musculus 
2022-09-29 | PXD037070 |
ProteomicsEvaluation and minimization of nonspecific tryptic cleavages in proteomic sample preparation
Dataset for evaluation of nonspecific cleavages and artificial modifications using E.coli and A549 cells
ORGANISM(S): Homo Sapiens Escherichia Coli 
2020-01-19 | PXD017186 |
ProteomicsThe exploration of Missing Proteins by a combination approach to enrich the low abundance membrane proteins from several cancer cell lines
Our goal is the identification of more and more the missing proteins from cancer cell lines. This project focuses on the membrance proteins separated by the ultracentrifuge and the low abundant peptides enriched by the Proteominer Kit and the hydrophobic peptides extracted by the organic solvent Ace...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-10-26 | MSV000086369 | MassIVE
ProteomicsGamma ray irradiation of epithelial carcinoma cells under hypoxic conditions
We demonstrate for the first time that under hypoxic conditions, A431 epithelial carcinoma cells exhibit increased viability when exposed to low-dose ?-irradiation, indicating that radiotherapy can promote tumor cell survival when oxygen supply is limited. When assessed using iTRAQ quantitative prot...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-29 | MSV000080784 | MassIVE
ProteomicsD283 MED, a cell line derived from peritoneal metastatic medulloblastoma — a good choice for missing protein discovery
Our goal is the identification of more and more missing proteins from cancer cell lines. This project focuses on D283 med and U-118mg cell lines which contribute to 12 missing protein identification.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-06-21 | MSV000087678 | MassIVE
ProteomicsHuman placental tissue LC-MS/MS
This project aimed at missing protein identification from human placental tissue, combining sample preparation and mass spectrometry technology development.For sample preparation, low molecular proteins were enriched by C18 solid phase extraction, SDS-PAGE and MWCO filter. For LC-MS/MS analysis, mic...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-10-29 | MSV000086385 | MassIVE
Proteomics[HPP-2.0]A simple, rapid, and practical method for single-cell proteomics based on mass-adaptive coating of synthetic peptides
Here, we report a simple, rapid, and practical approach of mass-adaptive coating-assisted single-cell proteomics (Mad-CASP). Combined with the designed synthetic peptides and novel data acquisition strategy, 1240 ± 93 (n = 3) proteins were identified from a single HeLa cell by Mad-CASP.
ORGANISM(S): Homo Sapiens 
2023-10-17 | PXD046177 |
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