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UnknownIron Oxide Nanoparticle-Mediated mRNA Delivery to Hard-to-Transfect Cancer Cells.
Not available
S-EPMC10384052 | biostudies-literature
UnknownHighly Efficient Messenger RNA Transfection of Hard-to-Transfect Cells using Carbon Nanodots.
Not available
S-EPMC10433478 | biostudies-literature
UnknownSuccessful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids.
Not available
S-EPMC7305135 | biostudies-literature
UnknownEffects of Decomplexation Rates on Ternary Gene Complex Transfection with α-Poly(l-Lysine) or ε-Poly(l-Lysine) as a Decomplexation Controller in An Easy-To-Transfect Cell or A Hard-To-Transfect Cell.
Not available
S-EPMC7356167 | biostudies-literature
UnknownSystemic Optimization of Gene Electrotransfer Protocol Using Hard-to-Transfect UT-7 Cell Line as a Model.
Not available
S-EPMC9687892 | biostudies-literature
ProteomicsDevelopment of nucleus-targeted synthetic histone photoaffinity probes
Dissection of the physiological interactomes of histone post-translational modifications (hPTMs) is crucial for understanding fundamental epigenetic regulatory pathways. Current peptide- or protein-based histone photoaffinity probes have greatly expanded the ability to probe the epigenetic interacto...
ORGANISM(S): Mus musculus (Mouse) 
2024-11-21 | PXD045620 | Pride
Nucleus-targeted histone photoaffinity probe Lactylation The epigenetic interactome Hard-to-transfect cells Native cells 
ProteomicsA click-compatible BmTyr platform for biotin-free proximity labeling and subcellular proteome profiling in primary T cells
Proximity labeling has revolutionized the study of dynamic subcellular proteomes by enabling the capture of transient protein interactions within living cells, yet the application of existing platforms to hard-to-transfect primary cells remains challenging. Here, we leverage a bioorthogonal proximit...
ORGANISM(S): Mus Musculus 
PXD078205 |
UnknownDeep Learning-Assisted Automated Single Cell Electroporation Platform for Effective Genetic Manipulation of Hard-to-Transfect Cells.
Not available
S-EPMC9119920 | biostudies-literature
ProteomicsProteome analysis of NanoMEDIC particles
CRISPR-Cas9 has tremendous potential as a therapeutic tool for treating human diseases. However, prolonged expression of the nuclease and gRNA from viral vectors in an in vivo context may cause off-target activity and immunogenicity. While extracellular vesicles have been recently demonstrated to be...
ORGANISM(S): Homo Sapiens (human) 
2020-01-30 | PXD014527 | JPOST Repository
ProteomicsA photo-oxidation driven proximity labeling strategy enables profiling of mitochondrial proteome dynamics in living cells
We herein report a photo-oxidation driven proximity labeling strategy to profile mitochondrial proteome by light dependence in living cells with high spatiotemporal resolution. Taking advantage of organelle-localizable organic photoactivated probe generating reactive species and nucleophilic substra...
ORGANISM(S): Homo sapiens (Human) 
2023-03-11 | PXD036630 | Pride
Photo-oxidation Proximity labeling Mitochondrial proteome 
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