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MetabolomicsMonitoring recombinant protein expression in bacteria by rapid evaporative ionisation mass spectrometry

RATIONALE: There is increasing interest in methods of direct analysis mass spectrometry that bypass complex sample preparation steps.

METHODS: One of the most interesting new ionisation methods is rapid evaporative ionisation mass spectrometry (REIMS) in wh...

2022-01-28 | MTBLS957 | MetaboLights
ProteomicsSynthetic biology meets proteomics: Construction of à la carte QconCATs for absolute protein quantification C4PR_LIV
We report a new approach to the assembly and construction of QconCATs, quantitative concatamers for proteomic applications that yield stoichiometric quantities of sets of stable isotope-labelled internal standards. The new approach is based on synthetic biology precepts of biobricks, making use of ...
ORGANISM(S): Escherichia coli 
2021-11-15 | PXD027521 | Pride
Synthetic biology Qconcat Quantitative proteomics Loop assembly 
ProteomicsStrategies to enhance the extraction of electrophoretically separated proteins from polyacrylamide gels and their application to mass spectrometry analyses (C4PR_LIV)
Polyacrylamide gel electrophoresis (PAGE) is a powerful technique for separating proteins extracted from complex biological samples. Unfortunately, the difficulty of recovering intact proteins in high yield from polyacrylamide matrices often limits further analyses. We discovered that staining prote...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) Drosophila melanogaster (Fruit fly) 
2020-07-13 | PXD010592 | Pride
Protein extraction Sds page 
ProteomicsPEPPI-MS- and GELFrEE-fractionated E. coli MG1655 whole cell lysate
Supplemental Files accompanying manuscript “PEPPI-MS: polyacrylamide gel-based prefractionation for analysis of intact proteoforms and protein complexes by mass spectrometry”.
ORGANISM(S): Escherichia Coli Str. K-12 Substr. Mg1655 (ncbitaxon:511145) 
2020-04-08 | MSV000085258 | MassIVE
ProteomicsSynthetic biology meets proteomics: Construction of à la carte QconCATs for absolute protein quantification
We report a new approach to the assembly and construction of QconCATs, quantitative concatamers for proteomic applications that yield stoichiometric quantities of sets of stable isotope-labelled internal standards. The new approach is based on synthetic biology precepts of biobricks, making use of l...
ORGANISM(S): Escherichia Coli 
2022-07-18 | PXD027047 | panorama
ProteomicsAbsolute Quantification of Schistosoma mansoni Esophageal Gland Products
Schistosomes are blood-dwelling helminth parasites causing a debilitating disease in the tropics. Major challenges to control the disease persist and vaccines would provide an additional tool, but their development has been problematic. Rhesus macaques can self-cure following schistosome infection, ...
ORGANISM(S): Schistosoma Mansoni 
2019-11-22 | PXD014899 | panorama
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