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ProteomicsE. coli S30 lysate proteome - The E
Protein production using processed cell lysates, defined precursor cocktails and target specific additives has become a core technology in the field of synthetic biology. Due to their open nature, these artificial systems are excellent to produce difficult proteins such as toxins or membrane protein...
ORGANISM(S): Escherichia coli 
2017-09-29 | PXD004646 | Pride
Qualitative and quantititative proteomics Heatshock lysate E. coli a19 S30 lysate 
ProteomicsYeast Heatshock SILAC LC-MSMS
Yeast cells were incubated at 25˚C, unless otherwise stated. For SILAC, cells were grown in light labeled (0.03mg/ml Lys0, 0.02mg/ml Arg0) or heavy labeled (0.03mg/ml Lys4, 0.02mg/ml Arg6) amino acid in synthetic defined medium with 2% dextrose for at least 7 generations. heat shock was performed i...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-06-12 | PXD012631 | Pride
Stress granule Yeast Heatshock 
ProteomicsEarly- and late-passage primary human mesemchymal stem cells subjected to 2 hour heat shock
Cells respond to stress by synthesising chaperone proteins that correct protein misfolding to maintain function. However, protein homeostasis is lost in ageing, leading to aggregates characteristic of protein-folding diseases. Whilst much is known about how these diseases progress, discovering what ...
ORGANISM(S): Homo sapiens (Human) 
2021-06-15 | PXD025280 | Pride
Human senescent heat shock msc mesenchymal stem cell 
ProteomicsHSPA1A inhibition in early- and late-passage primary human mesemchymal stem cells subjected to 2 hour heat shock
Cells respond to stress by synthesising chaperone proteins that correct protein misfolding to maintain function. However, protein homeostasis is lost in ageing, leading to aggregates characteristic of protein-folding diseases. Whilst much is known about how these diseases progress, discovering what ...
ORGANISM(S): Homo sapiens (Human) 
2021-06-15 | PXD025305 | Pride
Human senescent heat shock msc mesenchymal stem cell hsp70 inhibition hspa1a 
TranscriptomicsChIP-chip of E. coli K-12 MG1655 with antibody against RNA polymerase subunits, RpoB, RpoD, RpoS,RpoH, RpoN, FliA, and FecI
We investigate sigma factor binding regions for each sigma factor under various enviromental and/or genetic conditions. To measure sigma factor binding at a genome scale, we employed a ChIP-chip method to derivative strains of E. coli K-12 MG1655 wild type and its isogenic rpoS and rpoN knock-out s...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2014-02-05 | E-GEOD-46541 | biostudies-arrayexpress
ProteomicsThe UBA domain of Ubc1 (Ube2K) Facilitates the Assembly of K48/K63-Branched Ubiquitin Chains
The assembly of a specific polymeric ubiquitin (Ub) chain on a target protein is a key event in the regulation of numerous cellular processes. Yet, the mechanisms that govern the selective synthesis of particular polyubiquitin signals remain enigmatic. The ubiquitin-conjugating (E2) enzymes Ubc1 in ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2021-03-02 | PXD022621 | Pride
Ubiquitin Ubc1 Ubc4 Yeast Branched 
TranscriptomicsRNA polymerase
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2014-02-05 | E-GEOD-46740 | biostudies-arrayexpress
TranscriptomicsChIP-chip of Geobacter sulfurreducens PCA with antibody against various sigma factors under various conditions
We applied a ChIP-chip approach to elucidate the binding profiles of sigma factors (RpH, RpoN and RpoS) experimentally under different growth conditions. This technique localizes DNA fragments within DNA-protein complexes enriched by chromatin immunoprecipitation using high-density oligonucleotide t...
ORGANISM(S): Geobacter sulfurreducens PCA 
2013-05-01 | E-GEOD-37778 | biostudies-arrayexpress
ProteomicsIdentification of Proteins Sensitive to Thermal Stress in Human Neuroblastoma and Glioma Cell Lines
Heat-shock is an acute insult to the mammalian proteome. The sudden elevation in temperature has far-reaching effects on protein metabolism, leads to a rapid inhibition of most protein synthesis, and the induction of protein chaperones. Using heat-shock in cells of neuronal (SH-SY5Y) and glial (CCF-...
ORGANISM(S): Homo sapiens (Human) 
2016-04-11 | PXD003914 | Pride
Detergent insoluble Sh-sy5y Heatshock Ccf-sttg1 Cultured cell 
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