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TranscriptomicsNPAS3-HEK293-circadian-Illumina
The fragments of FLNPAS3 and DeltaNPAS3 were transferred into a similar TET-inducible expression plasmid and transfected into HEK293 [T-REx-293] cells (Invitrogen). At the zero hour time-point, cells were shifted into a serum-rich medium plus tetracycline in order to induce circadian cycling and NPA...
ORGANISM(S): Homo sapiens 
2011-10-03 | E-TABM-1079 | biostudies-arrayexpress
TranscriptomicsSNP microarray of T-REx-293-based clones overexpressing the DNA cytosine deaminase APOBEC3B
A conditional and isogenic system for APOBEC3B (A3B) induction in T-REx-293 cells. Cells were engineered to express doxycycline-inducible A3B-eGFP or eGFP constructs and subjected to 10 rounds of A3B-eGFP exposure causing 80-90% cell death. Control pools (eGFP) were subjected to parallel rounds of n...
ORGANISM(S): Homo sapiens 
2016-06-30 | E-GEOD-78710 | biostudies-arrayexpress
TranscriptomicsNPAS3-HEK293-standardcond-Illumina
NPAS3 or truncated NPAS3 overexpressed HEK293 cells or control HEK293 cells were collected at 24 hours after gene overexpression.Total RNA from alll samples was extracted.Biotinylated cRNA was prepared using the Illumina total Prep RNA application Kits.Hybridization, washing and scanning were perfor...
ORGANISM(S): Homo sapiens 
2011-10-03 | E-TABM-1078 | biostudies-arrayexpress
ProteomicsPhosphoproteome of PINK1 in HEK293 cells
triple SILAC phosphoproteomics experiment of Flp-In T-Rex HEK293 cells stably expressing either FLAG empty, PINK1-FLAG Kinase dead or PINK1-FLAG wild type were grown in ‘light’ (K0R0), ‘medium’ (K4R6) and ‘heavy’ (K8,R10) SILAC media, respectively.
ORGANISM(S): Homo sapiens (Human) 
2015-09-16 | PXD002127 | Pride
Pink1 Parkinson's disease Human Phosphorylation Phosphoproteomics Hek293 
TranscriptomicsRNA-Seq of WT Flp-In T-REx 293, UPF3A knockout and UPF3A overexpression cell lines in part with additional siRNA-mediated control or UPF3B knockdowns
UPF3A and UPF3B are paralogous genes in human cells that are involved in the nonsense-mediated decay (NMD) pathway. NMD is a cellular quality control mechanism that monitors mRNAs during translation. Aberrant translation due to features such as the presence of a premature stop codon downstream on an...
ORGANISM(S): Homo sapiens 
2022-08-31 | E-MTAB-10718 | biostudies-arrayexpress
TranscriptomicsiCLIP of TDP-43
A stable HEK293 FlpIn T-Rex cells expressing TDP-43 with an N-terminal eGFP-tag was generated that allowed inducible physiological expression of the protein (Ling et al. 2010). Duplicate iCLIP experiments were performed using an antibody targeting eGFP (Abcam ab290). Crosslinked RNA-protein complexe...
ORGANISM(S): Homo sapiens 
2017-05-01 | E-MTAB-4733 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human HNF1beta mutated vs wildtype embryonic kidney cell line HEK293 to identify potential HNF1beta target genes whose activity may be deregulated in human patients
Hepatocyte nuclear factor 1beta (HNF1beta, TCF2) is a tissue-specific transcription factor whose mutation in humans leads to renal cysts, genital malformations, pancreas atrophy and maturity onset diabetes of the young (MODY5). Furthermore, HNF1beta overexpression has been observed in clear cell can...
ORGANISM(S): Homo sapiens 
2007-09-07 | E-GEOD-3308 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human HEK293 cells overexpressing HNF4a2 wild type or mutant forms C106R or R154X
Identification of genes regulated by the transcription factor HNF4a2 Experiment Overall Design: We used microarrays to identify genes regulated by the transcription factor HNF4a2. HEK293 cell lines containing doxycyclin-inducible wilt type or mutant forms of HNF4a2m which were introduced by FRT/FLP ...
ORGANISM(S): Homo sapiens 
2008-10-18 | E-GEOD-8289 | biostudies-arrayexpress
TranscriptomicsGene expression profiling in HEK293 cells overexpressed lactoferrin gene that has the signal sequence (sLF) or not (ΔLF)
Human lactoferrin (LF) is a multifunctional protein involved in immunomodulation, cell growth, and differentiation. In addition to the secreted form (sLF), an alternatively spliced form (ΔLF) that lacks the signal sequence and downregulated in cancer was found. This study was carried out to identify...
ORGANISM(S): Homo sapiens 
2011-09-06 | E-GEOD-31878 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human HEK-293 cells to identify Dicer and Argonaute regulated gene expression
HEK-293-derived cell lines depleted of Dicer or one of four Argonaute proteins were generated. Microarray analysis of these knock down cell lines and various control cell lines was performed to address how large the proportion of genes is that are regulated by RNA silencing in an individual cell typ...
ORGANISM(S): Homo sapiens 
2008-06-12 | E-GEOD-4246 | biostudies-arrayexpress
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