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MetabolomicsIdentification of metabolites of trifunctional sphingomyelin (TFSM1) in HeLa 229 cells infected with Chlamydia trachomatis

Chlamydia trachomatis is an obligate intracellular bacterial pathogen and a leading cause of sexually transmitted infections worldwide. During its biphasic developmental cycle, infectious, non-replicative elementary bodies alternate with replicative reticulate bodies within a membrane-bound intra...

2026-08-10 | MTBLS15303 | MetaboLights
ProteomicsTargeted LC-MS/MS analysis of oSCRIB protein in HeLa cells.
LC-MS/MS analysis of small HeLa proteins (approximately 10–15 kDa) for detecting oSCRIB protein with Parallel Reaction Monitoring (PRM) mode.
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD027841 | Pride
Human Prm Hela Lc-ms/ms 
ProteomicsProteins associated with chromatin in Hela cells detected by LC-MS
Chromatin fraction was seperated from Hela cells. Proteins associated with chromatin was detected by LC-MS.
ORGANISM(S): Homo sapiens (Human) 
2024-11-15 | PXD053976 | Pride
Hela chromatin lc-ms 
ProteomicsProtein abundance estimation in HeLa cells
To estimate the amount of proteins in HeLa, whole cell digests were subjected to fractionation by off line reverse phase chromatography at basic pH followed by LC-MS/MS analysis
ORGANISM(S): Homo sapiens (Human) 
2016-12-07 | PXD001441 | Pride
Hela proteome Lc-ms/ms 
ProteomicsHeLa cell proteome: Different amounts of the tryptic peptide mixtures for LC-MS/MS
The tryptic peptide mixture from HeLa cells was subjected to LC-MS/MS in triplicate each for 250 ng, 500 ng, 750 ng and 1000 ng peptides. LC-MS/MS was conducted using a Q Exactive mass spectrometer (Thermo Fisher Scientific) equipped with an Ultimate 3000 RSLCnano liquid chromatograph (Thermo Fisher...
ORGANISM(S): Homo Sapiens (human) 
PXD013493 | JPOST Repository
UnknownOffline pentafluorophenyl (PFP)-RP prefractionation as an alternative to high-pH RP for comprehensive LC-MS/MS proteomics and phosphoproteomics.
Not available
S-EPMC5704945 | biostudies-literature
MetabolomicsProteomics and tracer metabolomics link GAPDH ISGylation to glycolytic control

Ubiquitin-like protein ISG15 (interferon-stimulated gene 15) has been implicated in the regulation of central carbon metabolism, but conflicting findings across experimental systems have limited mechanistic insight. Here, we applied a multi-omics approach in cells ectopically expressing the ISGyl...

2026-03-02 | MTBLS12619 | MetaboLights
ProteomicsLC-MS/MS of YfiD-interacting proteins in mammalian cells
To figure out the intracellular function of YfiD, HA-tagged YfiD or Mock was expressed in the HeLa cell line. Subsequently, the lysates of HeLa-YfiD or HeLa-Mock cells were immunoprecipitated with anti-HA beads followed by LC-MS/MS analysis.
ORGANISM(S): Homo sapiens (Human) 
2024-01-15 | PXD047656 | Pride
Lc-ms/ms Yfid Mammalian cells 
ProteomicsPipelines and Systems for Threshold Avoiding Quantification of LC-MS/MS data
The accurate processing of complex LC-MS/MS data from biological samples is a major challenge for metabolomics, proteomics and related approaches. Here we present the Pipelines and Systems for Threshold Avoiding Quantification (PASTAQ) LC-MS/MS pre-processing toolset, which allows highly accurate qu...
ORGANISM(S): Homo sapiens (Human) Escherichia coli Saccharomyces cerevisiae (Baker's yeast) 
2021-08-18 | PXD024584 | Pride
Lc-ms/ms pre-processing Isotope quantification Proteomics Label-free 
ProteomicsConfetti: A Multi-protease Map of the HeLa Proteome for Comprehensive Proteomics
Tryptic digestion proteomics gives limited coverage of many protein sequences as many proteins produce tryptic peptides that cannot be observed easily. Confetti is our comprehensive map of coverage for the HeLa proteome, using 48 different digests and CID/HCD LC-MS/MS analyses. An associated dataset...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-10-12 | MSV000081607 | MassIVE
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