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TranscriptomicsChanges in gene expression analysis of alphaCD3alpha/CD28 activated human naive and memory regulatory T cells (Treg) upon IL-7 treatment as measured by quantitative PCR array
This study evaluated changes in gene expression upon IL-7 treatment in human naive and memory Treg. CD4+CD25+CD127low naïve and memory Treg were isolated from fresh PBMC and separately stimulated with ?CD3?/CD28 coupled beads (Invitrogen-Dynal) at a 1:10 bead/T cell ratio, treated with or without 10...
ORGANISM(S): Homo sapiens 
2012-09-12 | E-GEOD-40755 | biostudies-arrayexpress
UnknownA set of gene knockouts as a resource for global lipidomic changes.
Enzyme specificity in lipid metabolic pathways often remains unresolved at the lipid species level, which is needed to link lipidomic molecular phenotypes with their protein counterparts to construct functional pathway maps. We created lipidomic profiles of 23 gene knockouts in a proof-of-concept st...
S-EPMC9218125 | biostudies-literature
TranscriptomicsGenome-scale RNAi profiling of cell division in human tissue culture cells
Cell division is a fundamental process for multicellula organisation. Here we report a genome-scale RNAi screen in HeLa cells designed to identify human genes important for cell division. We utilized a library of endoribonuclease-prepared siRNAs (esiRNAs) for gene-silencing and used DNA content an...
ORGANISM(S): Homo sapiens 
2010-06-25 | E-GEOD-9176 | biostudies-arrayexpress
UnknownCYP21A2 Gene Expression in a Humanized 21-Hydroxylase Mouse Model Does Not Affect Adrenocortical Morphology and Function.
Steroid 21-hydroxylase is an enzyme of the steroid pathway that is involved in the biosynthesis of cortisol and aldosterone by hydroxylation of 17α-hydroxyprogesterone and progesterone at the C21 position. Mutations in CYP21A2, the gene encoding 21-hydroxylase, cause the most frequent form of...
S-EPMC9113096 | biostudies-literature
UnknownComparative profiling identifies C13orf3 as a component of the Ska complex required for mammalian cell division.
Proliferation of mammalian cells requires the coordinated function of many proteins to accurately divide a cell into two daughter cells. Several RNAi screens have identified previously uncharacterised genes that are implicated in mammalian cell division. The molecular function for these genes needs ...
S-EPMC2684023 | biostudies-literature
UnknownHuman monocytes downregulate innate response receptors following exposure to the microbial metabolite n-butyrate.

Introduction

Hyporesponsiveness of human lamina propria immune cells to microbial and nutritional antigens represents one important feature of intestinal homeostasis. It is at least partially mediated by low expression of the innate response receptors CD11b, CD14, CD16 as well as the cystine...
S-EPMC5691313 | biostudies-literature
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