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GenomicsImpact of STAT5 dosage and binding location on genetic and biological programs activated in mouse mammary epithelium during pregnancy.
The transcription factor STAT5 has long been recognized as an important mediator of prolactin induced mammary development during pregnancy. We have used mouse genetics and genome-wide analyses to determine how altering concentrations of STAT5A and STAT5B impacts different target promoters. Examinati...
ORGANISM(S): Mus musculus 
2012-09-18 | E-GEOD-40930 | biostudies-arrayexpress
TranscriptomicsDistinct STAT5 concentrations uniquely control establishment and differentiation of mammary epithelium during pregnancy
To dissect regulatory processes of cell proliferation and differentiation we generated mouse strains carrying any combination of the four Stat5 alleles, thus expressing STAT5 from 0 to 100%. RNA-Seq analyses revealed that different STAT5 levels activate specific genetic programs linked to cell proli...
ORGANISM(S): Mus musculus 
2012-05-02 | E-GEOD-37646 | biostudies-arrayexpress
TranscriptomicsDevelopment of mammary luminal progenitor cells is controlled by the transcription factor STAT5A
Mammary alveologenesis is abrogated in the absence of the transcription factors STAT5A/5B that mediate cytokine signaling. To reveal the underlying causes for this developmental block we studied mammary stem and progenitor cells. While loss of STAT5A/5B did not affect the stem cell population and ...
ORGANISM(S): Mus musculus 
2009-10-21 | E-GEOD-17723 | biostudies-arrayexpress
TranscriptomicsGenome-wide analyses reveal the extent of opportunistic STAT5 binding that does not yield transcriptional activation of neighboring genes
Stat5+/- mice were bred into the C57BL/6 background. Stat5+/- mice were intercrossed and mouse embryonic fibroblasts (MEFs) were isolated from 12.5-13.5-day WT or Stat5-/- fetuses. The retroviral-expression vector carrying a wild-type Stat5A gene based on an MSCV-IRES-GFP backbone (gift from Richard...
ORGANISM(S): Mus musculus 
2012-03-14 | E-GEOD-33688 | biostudies-arrayexpress
TranscriptomicsThe gene encoding the hematopoietic stem cell regulator CCN3 (NOV) is under direct cytokine control through the transcription factors STAT5a/b.
Cytokines control the biology of hematopoietic stem and progenitor cells in part through the transcription factors STAT5a/b. CCN3/NOV has been reported as a positive regulator of hematopoietic stem and progenitor cells. We report microarray analyses of Lineage- Sca-1+ c-Kit+ (KSL) cells in the pre...
ORGANISM(S): Mus musculus 
2010-08-25 | E-GEOD-20684 | biostudies-arrayexpress
TranscriptomicsMiR-21 is targeted by STAT5 but dispensable for mammary gland development
In this study we identifies miR-21 to by under cytokine control through the transcription factor STAT5 and while miR-21 is differentially expressed during mammary gland development, miR-21 is dispensable for mammary development and lactation. We refer to wild-type mice (+/+) as WT and to mice lackin...
ORGANISM(S): Mus musculus 
2013-08-22 | E-GEOD-50068 | biostudies-arrayexpress
TranscriptomicsThe transcription factors STAT5a/b negatively regulate cell proliferation through the activation of cdkn2b and cdkn1a expression
Although the cytokine-inducible transcription factors STAT5a/b promote proliferation of a wide range of cell types, there are cell- and context specific cases in which loss of STAT5a/b results in enhanced cell proliferation. Here we report that loss of STAT5a/b from mouse embryonic fibroblasts (MEFs...
ORGANISM(S): Mus musculus 
2010-11-15 | E-GEOD-21861 | biostudies-arrayexpress
GenomicsLoss of EZH2 results in enhanced recruitment of EZH1, Pol II and Stat5 and precocious mammary gland development
Establishment and differentiation of mammary alveoli during pregnancy are controlled by prolactin through the transcription factor STAT5. As pregnancy progresses mammary signature genes are activated in a defined temporal order, which coincides with the recruitment of STAT5 to respective regulatory ...
ORGANISM(S): Mus musculus 
2015-01-27 | E-GEOD-65284 | biostudies-arrayexpress
GenomicsEZH2 controls mammary differentiation independently of its methyltransferase activity through controlling genomic STAT5 access
Mammary development is characterized by the proliferation and progressive differentiation of alveolar epithelium during pregnancy, culminating in lactation. These processes are largely controlled by hormones through transcription factors. We now explore the contributions of histone methyltransferase...
ORGANISM(S): Mus musculus 
2013-12-31 | E-GEOD-52016 | biostudies-arrayexpress
TranscriptomicsLoss of EZH2 results in precocious mammary gland development and the activation of STAT5-dependent genes
Establishment and differentiation of mammary alveoli during pregnancy are controlled by prolactin through the transcription factor STAT5. As pregnancy progresses mammary signature genes are activated in a defined temporal order, which coincides with the recruitment of STAT5 to respective regulatory ...
ORGANISM(S): Mus musculus 
2015-08-31 | E-GEOD-70440 | biostudies-arrayexpress
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