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MetabolomicsMetabolomics guides rational development of a simplified cell culture medium for drug screening against Trypanosoma brucei
In vitro culture methods underpin many experimental approaches to biology and drug discovery. The modification of established cell culture methods to be more biologically relevant, or to optimise growth, is traditionally a laborious task. Emerging metabolomics technology enables rapid evaluation of ...
2015-05-14 | MTBLS49 | MetaboLights
MetabolomicsMetabolomics revealed the influence of breast cancer on lymphatic endothelial cell metabolism, metabolic crosstalk, and lymphangiogenic signaling in co-culture

Breast cancer metastasis occurs via blood and lymphatic vessels. Breast cancer cells 'educate' lymphatic endothelial cells (LECs) to support tumor vascularization and growth. However, despite known metabolic alterations in breast cancer, it remains unclear how lymphatic endothelial cell metabolis...

2024-06-25 | MTBLS859 | MetaboLights
TranscriptomicsExpression data from human CD4 or CD8 T-cells isolated from PBMC cultured at a low cell density (LDC) or high cell density (HDC)
Research in human immunobiology is mainly based on working with peripheral blood mononuclear cells (PBMC). However, recent investigations have shown that circulating CD4+ T cells are less sensitive to several T-cell activating monoclonal antibodies (mAb) and to recall antigens as compared to tissue-...
ORGANISM(S): Homo sapiens 
2015-06-09 | E-GEOD-51288 | biostudies-arrayexpress
ProteomicsStress-Induced Premature Senescence in High Five Cell Cultures: A Principal Factor in Cell-Density Effects
The Baculovirus Expression Vector System (BEVS) is highly valued in vaccine development, protein engineering, and drug metabolism research due to its biosafety, operational convenience, rapid scalability, and capacity for self-assembling virus-like particles. However, increasing cell density at the ...
ORGANISM(S): Trichoplusia Ni 
2024-10-31 | PXD057379 |
ProteomicsExoproteome in a perfusion bioreactor containing antibody-producing CHO cells at various cell densities
Chinese Hamster ovary (CHO) cells are the main platform used to produce recombinant proteins in the biopharmaceutical industry and are commonly cultured in either fed-batch or perfusion mode. However, the optimization of the complex biological systems used in such processes is extremely challenging....
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2018-07-04 | PXD008760 | Pride
Perfusion Bioreactor High cell density Monoclonal antibody (mab) Chinese hamster ovary (cho) Exoproteome Lc-ms/ms 
TranscriptomicsTranscription profiling of E. coli high-cell-density recombinant and wild-type
The transcriptome profiles for wild-type (plasmid-free) and recombinant (plasmid-bearing) Escherichia coli during well-controlled synchronized high-cell-density fed-batch cultures were analyzed by DNA microarrays. It was observed that the growth phase significantly affected the transcriptome profile...
ORGANISM(S): Escherichia coli 
2009-08-25 | E-GEOD-17505 | biostudies-arrayexpress
TranscriptomicsGene expression profiles in semi-continuous culture with high-cell density under normal and low temperature conditions for scFv-Fc-producer CHO cells harboring an artificial promoter system with transcriptional positive-feedback loop of transactivator
We developed an artificial gene expression system with transcriptional positive-feedback loop of transactivator to induce the high expression of target genes in CHO cells. DNA microarray analysis was conducted in semi-continuous culture with high-cell-density culture under normal and low temperature...
ORGANISM(S): Cricetulus griseus 
2024-01-01 | E-MTAB-13440 | biostudies-arrayexpress
MetabolomicsLC-MS metabolic profiling of mouse plasma and cell culture medium in relation to ABCC6 expression
Pseudoxanthoma elasticum (PXE) is an autosomal recessive disease characterized by progressive ectopic mineralization of the skin, eyes and arteries, for which no effective treatment exists. PXE is caused by inactivating mutations in the gene encoding ABCC6 (MRP6), an ATP-dependent efflux transporter...
2013-11-14 | MTBLS61 | MetaboLights
TranscriptomicsTranscription profiling of Density-dependent of Xanthomonas oryzae pv. oryzae PXO99A and PXO99∆ax21
Xanthomonas oryzae pv. oryzae strain PXO99A, so called Xoo, is disable to infect in rice cultivar carrying Xa21 gene. A set of experiments indicated that Ax21 is quorum sensing factor in PXO99 strain. To observe fine-tuned details how Ax21 controls expression of PXO99 in response to change of cell p...
ORGANISM(S): Xanthomonas oryzae pv. oryzae PXO99A 
2012-01-01 | E-GEOD-24989 | biostudies-arrayexpress
TranscriptomicsGlobal transcriptome response of recombinant protein xylanase producing E. coli in high cell density fed batch culture
Strong production of recombinant proteins interfere with cellular processes in many ways. The extent of the bacterial stress response is determined by the specific properties of the recombinant protein, and by the rates of transcription and translation. The consideration of bacterial stress and star...
ORGANISM(S): Escherichia coli 
2011-05-23 | E-GEOD-29439 | biostudies-arrayexpress
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