Sort   by:  
 Page size 
MetabolomicsComparison of popular enrichment methods for untargeted in vitro metabolomics.

Introduction

Untargeted metabolomics is a popular method by which researchers measure a large portion of the metabolites present in a biological system at once. This approach usually results in complex data sets containing tens to hundreds of thousands of observations whi...

2025-08-11 | MTBLS12332 | MetaboLights
MetabolomicsStreamlined identification of strain engineering targets for bioprocess improvement using metabolic pathway enrichment analysis
Metabolomics is a powerful tool for the identification of genetic targets for bioprocess optimisation. However, in most cases, only the biosynthetic pathway directed to product formation is analysed, limiting the identification of these targets. Some studies have used untargeted metabolomics, allowi...
2023-08-31 | MTBLS6667 | MetaboLights
ProteomicsHILIC and ERLIC Enrichment of Glycopeptides Derived from Breast and Brain Cancer Cells
Aberrant glycosylation has been linked to many different cancer types. The blood brain barrier (BBB) is a region of the brain that regulates the entrance of ions, diseases, toxins, etc. However in breast cancer metastasis, the BBB fails to prevent the crossing of the cancer cells into the brain. Her...
ORGANISM(S): Homo sapiens (Human) 
2019-02-27 | PXD004361 | Pride
Hilic enrichment Glycoproteomics Erlic enrichment Mass spectrometry Blood brain barrier Cancer cell lines N-linked glycosylation 
MetabolomicsHelicobacter pylori infection promotes TMAO accumulation through Escherichia coli enrichment with gut–liver circadian disruption and hepatic metabolic injury
Helicobacter pylori (H. pylori) infection is associated with metabolic dysfunction-associated steatotic liver disease, but the microbial changes and circadian disturbances accompanying distal hepatic injury remain unclear. In specific pathogen-free (SPF) mice exposed to dietary choline, H. pylori in...
2026-09-18 | MTBLS15673 | MetaboLights
ProteomicsIntegrated Single-Tip IMAC-HILIC Enrichment Enables Simultaneous and Efficient Analysis of Plant Phosphoproteomics and N-glycoproteomics
Protein phosphorylation and N-glycosylation are key post-translational modifications (PTMs) in plants that regulate critical signaling processes. However, analyzing both PTMs simultaneously remains challenging due to low abundance and divergent enrichment requirements. Here, we present a single-tip ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-05-14 | PXD061247 | Pride
Phosphoproteomics  n-glycoproteomics  enrichment  immobilized metal affinity chromatography  hydrophilic interaction chromatography 
ProteomicsHILIC enrichment of Leishmania major MHOM
The trypanosomatid protozoan parasite Leishmania has a significant impact on human health globally. Understanding the pathways associated with virulence within this significant pathogen is critical for identifying novel vaccination and chemotherapy targets. Within this study we leverage an ultradeep...
ORGANISM(S): Leishmania major species complex 
2022-10-13 | PXD035738 | Pride
Leishmania major mhom Glycopeptides 
ProteomicsEvaluation of absorbent cotton for glycopeptide enrichment
This study introduced the use of cotton in 200µL tips, namely cotton HILIC as a convenient tool for enriching large-scale glycopeptides before MS analysis in this study. The optimal loading buffer for cotton HILIC was investigated by using mouse brain as a complex test sample. Subsequently, the perf...
ORGANISM(S): Mus musculus (Mouse) 
2023-03-04 | PXD035078 | Pride
Hilic Glycopeptide Enrichment absorbent cotton Mass spectrometry Max 
ProteomicsExploring the glycosylation state of the Cryptosporidium parvum CpTSP protein family-HILIC glycopeptide enrichment
Cryptosporidium parvum is a zoonotic apicomplexan parasite and a common cause of diarrheal disease worldwide. The development of vaccines to prevent or limit infection remains an important goal for tackling these diarrheal diseases, which are a significant cause of infant morbidity in the developing...
ORGANISM(S): Cryptosporidium parvum Iowa II 
2023-01-12 | PXD023936 | Pride
Cryptosporidium Glycosylation Thrombospondin repeat 
ProteomicsComparison of Intact Glycopeptide Enrichment Methods Using Strong Anion Exchange and Hydrophilic Interaction Liquid Chromatography
Heterogeneity of protein glycosylation poses great challenge for analysis that is key to un-puzzle systems glycobiology in diseases. Resolving this conundrum requires global enrichment of glycopeptides for identification and quantitation. To this aim, hydrophilic interaction chromatography (HILIC) h...
ORGANISM(S): Homo sapiens (Human) 
2017-10-19 | PXD005145 | Pride
Glycoproteomics Hilic Sax Quantitation O-linked 
UnknownHILIC and ERLIC Enrichment of Glycopeptides Derived from Breast and Brain Cancer Cells.
Not available
S-EPMC5748158 | biostudies-literature
Sort   by:  
 Page size