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ProteomicsMetabolic differences between morphologically distinct symbiont populations in the tubeworm Riftia pachyptila
The endosymbiont population of the hydrothermal vent tube worm Riftia pachyptila consists of a single 16S phylotype of sulfur-oxidizing gammaproteobacteria. The intracellular symbiont exhibits remarkable morphological heterogeneity, from small rod-shaped or coccoid cells to large cocci, which were s...
ORGANISM(S): Candidatus Endoriftia persephone Riftia pachyptila 
2020-12-21 | PXD016986 | Pride
Mutualism Metaproteomics Symbiosis Deep sea Hydrothermal vents Holobiont Gradient centrifugation Mutualistic association 
ProteomicsHost-symbiont interactions in the chemosynthetic Riftia model symbiosis
The deep-sea tubeworm Riftia pachyptila is a model system for a mutualistic association: The adult worm has no digestive system, but completely relies on one phylotype of endosymbiotic chemosynthetic bacteria for nutrition. The bacteria, in turn, are provisioned by the host. Metabolism and physiolog...
ORGANISM(S): Candidatus Endoriftia persephone Riftia pachyptila 
2019-11-25 | PXD012439 | Pride
Mutualism Metaproteomics Deep sea Hydrothermal vents Holobiont Mutualistic association 
ProteomicsMore is not always better: Evaluation of 1D- and 2D-LC-MS2 methods for metaproteomics
Metaproteomics is a powerful tool to characterize the structure of microbial communities and the physiology, metabolism and interactions of the species in these communities. Metaproteomics seeks to identify and quantify proteins from microbial communities on a large scale using gel electrophoresis o...
ORGANISM(S): Eukaryota (eucaryotes) Bacteria Archaea Viruses 
2019-02-12 | PXD008017 | Pride
Microbial community  microbiota  microbiome  mock community  method evaluation  microbial ecology  q exactive  liquid chromatography  gelc 
ProteomicsThe Trichoplax H2 holobiont: a simple animal and its two intracellular symbionts
The Placozoa are an enigmatic group of simple marine metazoans where all taxa harbor intracellular bacteria. Despite four decades of research, the bacterial identities, their location and their roles remain elusive. Here we show that the placozoan Trichoplax H2 is associated with two intracellular b...
ORGANISM(S): Trichoplax sp. H2 Candidatus Midichloriaceae Candidatus Margulisbacteria 
2019-04-18 | PXD012106 | Pride
Grellia incantans Rough endoplasmic reticulum Trichoplax h2 Ruthmannia eludens Placozoa Intracellular symbiosis Midichloriaceae Rickettsiales Margulisbacteria 
ProteomicsMetaproteomics and direct Protein-SIF of the deep-sea mussel Bathymodiolus childressi and its bacterial symbionts
Bathymodiolus childressi is a species of deep-sea mussels found predominantly in the Gulf of Mexico. It colonizes cold seeps such as brine pool and oil seeps. The success of these animals in such environment is thought to be due to the symbiotic association of the mussel host with several species of...
ORGANISM(S): Bacteria Bathymodiolus childressi Archaea Viruses 
2020-02-27 | PXD008089 | Pride
Stable isotope fingerprinting Chemosynthetic symbiosis Stable carbon isotopes 2d-lc-ms/ms Bacteria-animal symbiosis Q exactive plus Methanotrophic symbionts Cold seeps Epsilonproteobacteria Calvin cycle 1d-lc-ms/ms 
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