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ProteomicsEnhanced validation of antibodies for research applications
There is a need for standardized validation methods for antibody specificity and selectivity. Recently, five alternative validation “pillars" were proposed to explore the specificity of research antibodies using methods with no need for prior knowledge about the protein target. Here, we show that th...
ORGANISM(S): Homo sapiens (Human) 
2018-08-21 | PXD010178 | Pride
Western blot Sds-page U251 Rt4 Lc-msms 
ProteomicsTargeted Proteomics Using Stable Isotope Labeled Protein Fragments Enables Precise and Robust Determination of Total Apolipoprotein(a) in Human Plasma
Lipoprotein(a), also known as Lp(a), is an LDL-like particle composed of apolipoprotein(a) (apo(a)) bound covalently to apolipoprotein B100. Plasma concentrations of Lp(a) are highly heritable and vary widely between individuals. Elevated plasma concentration of Lp(a) is considered as an independent...
ORGANISM(S): Homo Sapiens 
2023-02-17 | PXD026976 | panorama
ProteomicsQuantitative assessment of a novel device designed for patient-centric sampling of dried plasma using targeted proteomics
The advancements in technology have significantly enhanced proteomics by improving the quality, depth, and timeliness of proteomic data, making mass spectrometry a promising tool for personalized medicine. To enable successful longitudinal population-level monitoring, it is essential to have cost-ef...
ORGANISM(S): Homo Sapiens 
PXD056596 | panorama
ProteomicsAbsolute Quantification of Apolipoproteins Following Treatment with Omega-3 Carboxylic Acids and Fenofibrate Using a High Precision Stable Isotope-Labeled Recombinant Protein Fragments Based SRM Assay
Stable isotope-labeled standard (SIS) peptides are used as internal standards in targeted proteomics to provide robust protein quantification, which is required in clinical settings. However, SIS peptides are typically added post trypsin digestion and, as the digestion efficiency can vary significan...
ORGANISM(S): Homo Sapiens 
PXD013314 | panorama
ProteomicsHigh throughput generation of a resource of the human secretome in mammalian cells
The proteins secreted by human tissues (the secretome) are important for the basic understanding of human biology, but also for identification of potential targets for future diagnosis and therapy. Here, we present an annotation of all predicted secreted proteins (n=2,623) with information about the...
ORGANISM(S): Homo Sapiens 
2021-03-15 | PXD012054 | panorama
ProteomicsRapid and Sensitive Detection of SARS-CoV-2 Infection Using Quantitative Peptide Enrichment Analysis
Reliable, robust, large-scale molecular testing for SARS-CoV-2 is essential for monitoring the ongoing Covid-19 pandemic. We have developed a scalable analytical approach to detect viral proteins based on peptide immunoaffinity enrichment combined with liquid chromatography - mass spectrometry (LC-M...
ORGANISM(S): Homo Sapiens Severe Acute Respiratory Syndrome Coronavirus 2 
2021-11-15 | PXD026366 | panorama
ProteomicsLongitudinal Protein Profiling using Targeted Proteomics and Recombinant Standards
Spike-in of standards of known concentrations used in proteomics-based workflows is an attractive approach for both accurate and precise multiplexed protein quantification. Here, a quantitative method based on targeted proteomics analysis of plasma proteins using isotope-labelled recombinant standar...
ORGANISM(S): Homo Sapiens 
2021-03-15 | PXD020797 | panorama
ProteomicsAddressing the Protease Bias in Quantitative Proteomics
Protein quantification strategies using multiple proteases have been shown to deliver poor inter-protease accuracy in label-free mass spectrometry experiments. By utilizing six different proteases with different cleavage sites, this study explores the protease bias and its effect on accuracy and pre...
ORGANISM(S): Homo Sapiens 
2022-09-02 | PXD033574 | panorama
ProteomicsAssessing the role of trypsin in quantitative plasma- and single-cell proteomics towards clinical application
Mass spectrometry-based bottom-up proteomics is rapidly evolving and routinely applied in large biomedical studies. Proteases are a central component of every bottom-up proteomics experiment, digesting proteins into peptides. Trypsin has been the most widely applied protease in proteomics, due to it...
ORGANISM(S): Homo Sapiens 
PXD042450 | panorama
ProteomicsPan-cancer plasma proteome profiling using SIS recombinant protein fragments reveals downregulation of proteins interacting with immunoglobulins in multiple myeloma
Mass spectrometry based on Data Independent Acquisition (DIA) strategies has developed into powerful tools suitable for precision medicine applications. Assays performed in this setting provide confident protein identification and precise quantification on an absolute scale when combined with stable...
ORGANISM(S): Homo Sapiens 
PXD037946 | panorama
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