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ProteomicsBovine protein glycation sites in different milk products
Here we studied the glycation of bovine milk proteins by lactose as dominant sugar in milk and hexoses using tandem mass spectrometry (CID and ETD mode). In a bottom-up proteomics approach after enriching glycated peptides by boronate affinity chromatography, first we could identify 260 lactosylated...
ORGANISM(S): Bos taurus (Bovine) 
2016-01-11 | PXD003054 | Pride
Electron transfer dissociation (etd) Infant formula Maillard reaction Boronate affinity chromatography (bac) Lactose-free milk Nrpc-esi-ms/ms Non-enzymatic glycosylation 
ProteomicsA workflow towards the reproducible identification and quantitation of protein carbonylation sites in human plasma
Protein carbonylation has been consistently used as a marker of excessive oxidative stress and studied in the context of multiple human oxidative stress related diseases. The variety of carbonyl post-translational modifications (PTMs) and their low abundance on easily accessible diagnostic tissues (...
ORGANISM(S): Homo sapiens (Human) 
2021-03-02 | PXD023738 | Pride
Protein carbonylation Lc-twims-ms/ms Aldehyde reactive probe (arp) Human plasma Biotin-avidin affinity 
UnknownRINT1 Bi-allelic Variations Cause Infantile-Onset Recurrent Acute Liver Failure and Skeletal Abnormalities.
Not available
S-EPMC6612521 | biostudies-literature
MetabolomicsLipidHunter Identifies Phospholipids by High-Throughput Processing of LC-MS and Shotgun Lipidomics Datasets (Rat cardiomyocyte assay)
Lipids are dynamic constituents of biological systems, rapidly responding to any changes in physiological conditions. Thus, there is a large interest in lipid-derived markers for diagnostic and prognostic applications, especially in translational and systems medicine research. As lipid identificatio...
2018-07-31 | MTBLS483 | MetaboLights
TranscriptomicsIdentification of KSHV, EBV and cellular miRNA targets in human B-cells using RIP-Chip
RIP-Chip was performed on DG75-eGFP, DG75-10/12, BCBL-1, BL41, BL41 B95.8 and Jijoye using anti-human Ago2 (11A9) antibodies. Anti-BrdU antibodies were used as controls for DG75-eGFP, DG75-10/12 and BCBL-1. Total RNA was used as control for BL41, BL41 B95.8 and Jijoye. Samples were analyzed on Affym...
ORGANISM(S): Homo sapiens 
2011-02-04 | E-GEOD-17180 | biostudies-arrayexpress
TranscriptomicsTGF-beta1-Induced Gene Expression Changes in HepG2 Cells
We here compared gene expression profiles in HepG2 cells upon stimulation with 1 ng/ml TGF-beta1 for 20 min, 1 hour, 2 hours, 4 hours, and 24 hours with untreated control cells. Experiments were done in three independent replicates. The goal of this study was to determine genes regulated by TGF-beta...
ORGANISM(S): Homo sapiens 
2013-04-10 | E-GEOD-45945 | biostudies-arrayexpress
TranscriptomicsTGF-beta1-Induced Gene Expression Changes in Primary Murine Hepatocytes
We here compared gene expression profiles of primary murine hepatocytes (mPC) upon stimulation with 1 ng/ml TGF-beta1 for 20 min, 2 hours and 4 hours with untreated cells. Experiments were done in three independent replicates. The goal of this study was to determine genes regulated by TGF-beta1. 12 ...
ORGANISM(S): Mus musculus 
2013-04-10 | E-GEOD-45942 | biostudies-arrayexpress
ProteomicsScreening of protein carbonylation sites in human serum by ion mobility mass spectrometry
Excessive oxidative stress, which is associated with several diseases, can trigger oxidative protein modifications, including protein carbonylation, which refers to the oxidative formation of aldehyde or keto groups in proteins. The structural diversity of these non-enzymatic reactive carbonyl group...
ORGANISM(S): Homo Sapiens 
PXD058666 | panorama
ProteomicsIn-Gel digest and filter-aided sample preparation to identify Dermatophagoides - and yeast protein antigens in severe equine asthma.
Severe equine asthma (SEA) is a common, chronic respiratory disease of horses characterized by hyperreactivity to hay dust which has many similarities to severe neutrophilic asthma in humans. A 2D immunoblot approach was used to identify quantitative differences in the recognition of the two-dimensi...
ORGANISM(S): Dermatophagoides Pteronyssinus Saccharomyces Cerevisiae 
2024-01-26 | PXD045568 | panorama
ProteomicsImproving peptide fragment ion detection using travelling wave ion mobility spectrometry with signal enhancement MSE (seMSE)
Recently, a solution to inherent poor ion beam sampling of time-of-flight (TOF) mass analyzers was proposed by restricting detection to ions expected at specific arrival times after being separated in a travelling wave ion mobility spectrometry (TWIMS) cell. Here, we show that this approach can be a...
ORGANISM(S): Oryctolagus Cuniculus Escherichia Coli Bos Taurus Saccharomyces Cerevisiae 
PXD030431 | panorama
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