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Analysis of cryopreservation effects on peripheral blood mononuclear cells at gene expression level. The hypothesis tested in the present study was that cryopreservation has an influence on the transcriptome profile of peripheral blood mononuclear cells. Results indicated remarkable changes in expre...
ORGANISM(S): Homo sapiens 
Analysis of long-term freezing on the stability of transcriptome profiles in PAXgene stabilized whole blood samples. In the present study it was tested if long-term freezing of PAXgene RNA tubes (up to one year) has an influence on the transcriptome profile of peripheral whole blood samples. Resul...
ORGANISM(S): Homo sapiens 
Calu-3 cells are human lung cancer cells that serve as a model of respiratory epithelial cell model. Here, Calu-3 cells are used as a cell system for Middle East respiratory syndrome coronavirus and treated with Cyclosporin A To assess the effect of Cyclosporin A on the host cells, uninfected cells ...
ORGANISM(S): Homo sapiens 
To analyse the influence of pentanoate on the differentiation of naive CD4 T cells under Th17 inducing conditions, murine CD4 T cells were isolated from spleen and lymphnodes of FIR/TIGER mice (IL-10 (GFP) - Foxp3 (RFP) - Reporter mice). The T cells were kept for 3 days under Th17 inducing condition...
ORGANISM(S): Mus musculus 
We tested the influence of pentanoate on B cells by treatment of CpG-stimulated B cells in vitro. We isolated naive B cells from FIR/TIGER mice (IL-10 (GFP) - Foxp3 (RFP) - Reporter mice) from spleen. Cells were cultured in presence of 5 µg/ml CpG 2395. 24h after CpG-stimulation, B cells were treate...
ORGANISM(S): Mus musculus 
The X-chromosomal dystonia parkinsonism syndrome (XDP) is associated with sequence changes within the TAF1/DYT3 multiple transcript system. While most sequence changes are intronic, one, DSC3, is located within an exon (d4). Transcribed exon d4 occurs as part of multiple splice variants. These varia...
ORGANISM(S): Homo sapiens 
Gene expression arrays and genotyping data in primary human monocytes from healthy individuals of European ancestry that have either been unstimulated (baseline), exposed to Lipopolysaccharide (90 minutes or 6 hours duration), muramyl-dipeptide (90 minutes or 6 hours duration) or transfected with 5’...
ORGANISM(S): Homo sapiens 
Since radiotherapy is the essential treatment of prostate cancer, this experiment aimed to elucidate the transcriptomic response of prostate cancer cells to irradiation by either conventional photons or alternatively carbon ions, focusing on DNA damage, DNA repair and androgen receptor signaling.
ORGANISM(S): Homo sapiens 
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