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Nitrogen cavitation of bovine sperm to isolate plasma membrane. FASP digestion using trypsin. Data acquired using 5 workflows: (1) Unfractionated on a 5600 TripleTOF; (2) SCX-ESI-LC-MS/MS on a QSTAR Elite; (3) 1D Gel-LC-ESI-LC-MS/MS on a QSTAR Elite; (4) SCX-LC-MALDI-MS/MS on a UltrafleXtreme; (5) ...
ORGANISM(S): Bos taurus (Bovine) 
2020-01-27 | PXD000007 | Pride

Ferroptosis is mediated by lipid peroxidation of phospholipids containing polyunsaturated fatty acyl moieties. Glutathione, the key cellular antioxidant capable of inhibiting lipid peroxidation via the activity of the enzyme glutathione peroxidase 4 (GPX-4), is generated directly from the sulfur-...

2023-01-11 | MTBLS5959 | MetaboLights
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