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Honeybee semen was collected by gently squeezing the male abdomens. Samples were pooled and then centrifuged. Pelleted sperm was collected and analysed with both 2D PAGE and gel-free methods. For the 2D PAGE, protein spots were digested using trypsin. Extracted peptides, resolved on a C18 column, we...
ORGANISM(S): Apis mellifera (Honeybee) 
2020-01-17 | PXD000169 | Pride
Experiment was designed to study the effect of Deformed wing virus (DWV) and the mite Varroa destructor on global gene expression using microarray transcriptional profiling in developing worker honeybee (Apis mellifera). Newly hatched bee larvae (day 3 of bee development) were transferred from a Var...
ORGANISM(S): Apis mellifera 
Honeybees are very important eusocial insects and are involved in the pollination of many plants. Queen bees and worker bees develop from the same fertilized eggs, and are thus genetically identical despite their substantial behavioural and physiological differences. The mechanism governing developm...
ORGANISM(S): Apis mellifera 
The honeybee (Apis mellifera L.) is a eusocial insect that uses various chemical signals such as royal jelly (RJ) produced by the worker exocrine glands, to maintain the ‘physiology’ of its colony. The roles of worker hypopharyngeal glands (HpG) and the changes they undergo according to the division...
ORGANISM(S): Cellular Organisms 
In honeybees, division of labour is a key feature, with age-related behavioural transitions being closely associated with molecular changes in the brain, gut, and microbiota. In this study, we integrated omics approaches to describe the global metabolomic and proteomic shifts in honeybee workers and...
ORGANISM(S): Apis mellifera (Honeybee) 
2026-02-27 | PXD057060 | Pride
The honeybee brain is comprised of a nervous system that sufficiently regulates this life transition. Knowledge about how protein phosphorylation functions in regards to the neurobiological activities in the honeybee brain to drive the age-specific labor division is still lacking. Protein phosphoryl...
ORGANISM(S): Apis mellifera (Honeybee) 
2018-10-26 | PXD003757 | Pride
To explore brain neuropeptidic functions in behavioral regulation, a label-free quantitative strategy was employed to compare neuropeptidomic variations between behavioral phenotypes (nurse bees, nectar foragers, and pollen foragers) and the two honeybee species (Apis mellifera ligustica and Apis ce...
ORGANISM(S): Apis mellifera (Honeybee) 
2021-09-09 | PXD018713 | Pride
Experiment was designed to study the effect of Deformed wing virus (DWV) and the mite Varroa destructor on on siRNA and miRNA composition using high-throughput sequencing of small RNA in developing worker honeybee (Apis mellifera). Newly hatched bee larvae (day 3 of bee development) were transferre...
ORGANISM(S): Apis mellifera 
he brain is a vital organ in regulating complex social behaviors of honeybees including learning and memory. Knowledge of how brain membrane proteins and their phosphorylation underlie the age-related behavioral polyethism is still lacking. We presented the first comprehensive profiling and comparis...
ORGANISM(S): Apis mellifera (Honeybee) 
2017-09-11 | PXD006823 | Pride
Royal jelly (RJ) is a proteinaceous secretion of the hypopharyngeal glands (HGs) in the head of honeybee workers. It is a critical food for queen bees and young larvae that decides the fate of fertilized eggs in developing into either queen bees or worker bees during the early larval stages. RJ is a...
ORGANISM(S): Apis mellifera (Honeybee) 
2019-01-11 | PXD010898 | Pride
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