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ProteomicsRapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling
Membrane proteins are crucial targets for cancer biomarker discovery and drug development. However, in addition to the inherent challenges of hydrophobicity and low abundance, complete membrane proteome coverage of clinical specimen is usually hindered by the requirement of large amount of starting ...
ORGANISM(S): Rattus rattus (Black rat) Homo sapiens (Human) 
2015-11-16 | PXD001548 | Pride
High-ph reverse phase stagetip Membrane proteins Lung cancer 
ProteomicsMining Missing Membrane Proteins by High-pH Reverse Phase StageTip Fractionation and Validation by Multiple Reaction Monitoring Mass Spectrometry
Several significant efforts have been made in the past decade to map all human proteome. However, more than 3000 proteins are grouped as missing having no evidence of translation. Membrane protein annotation of the current missing proteins list showed 34%to be missing membrane proteins, which are ch...
ORGANISM(S): Homo sapiens (Human) 
2015-07-27 | PXD002224 | Pride
High-ph reverse phase Stagetip Missing proteins Lung cancer 
ProteomicsRapid High-pH Reverse Phase StageTip for Sensitive Small-Scale Membrane Proteomic Profiling
To increase the coverage of membrane protein identification,we developed high-pH reverse phase (Hp-RP) combined with stop-and-go extraction tip (StageTip) technique, as a fast (∼15 min.). Almost 5000 proteins (∼60% membrane proteins) can be identified in only 10 μg of membrane protein digests, showi...
ORGANISM(S): Homo Sapiens (human) 
2016-09-08 | PXD004904 | JPOST Repository
UnknownMining Missing Membrane Proteins by High-pH Reverse-Phase StageTip Fractionation and Multiple Reaction Monitoring Mass Spectrometry.
Not available
S-EPMC4761227 | biostudies-literature
ProteomicsMouse cardiomyocytes LC/MS/MS - Membrane Proteomics of Impaired Energetics and Cytoskeletal Disorganization in Elderly Diet-Induced Diabetic Mice
Differential Membrane Proteomics Profiles of isolated cardio myocytes in HFD mice
ORGANISM(S): Mus musculus (Mouse) 
2017-08-28 | PXD002881 | Pride
Hp-rp stagetip fractionation Lc/ms/ms Mouse cardiomyocytes Itraq labeling 
ProteomicsHs-CHPP_LungCancer-Hp-RP-StageTip_CL141
Mining Missing Membrane Proteins, Hp-RP StageTips, CL141
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005374 | PeptideAtlas
ProteomicsHs-CHPP_LungCancer-Hp-RP-StageTip_CL97
Mining Missing Membrane Proteins, Hp-RP StageTips, CL97
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005449 | PeptideAtlas
ProteomicsHs-CHPP_LungCancer-Hp-RP-StageTip_A549
Mining Missing Membrane Proteins, Hp-RP StageTips, A549
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005358 | PeptideAtlas
ProteomicsHs-CHPP_LungCancer-Hp-RP-StageTip_H3255
Mining Missing Membrane Proteins, Hp-RP StageTips, H3255
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005336 | PeptideAtlas
ProteomicsHs-CHPP_LungCancer-Hp-RP-StageTip_PC9
Mining Missing Membrane Proteins, Hp-RP StageTips, PC9
ORGANISM(S): Homo Sapiens (human) 
2015-12-31 | PAe005379 | PeptideAtlas
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