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Plant phosphoproteomics reveals a global view of phosphorylation-mediated signaling in plants, but it remains demanding a high-throughput method with detection sensitivity and quantification accuracy. In general, the indispensable protein precipitation step requiring for interferences removal limite...
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
We developed a tip-based sequential IMAC based on different kinds of metal ion for increasing the coverage of phosphoproteome. This approach not only can be used for singly and multiply phosphopeptides separation but also for acidic, basophilic and Pro-directed phosphopeptides separation.
ORGANISM(S): Homo Sapiens (human) 
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