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ProteomicsDeep Coverage and Extended Sequence Reads Obtained with a Single Archaeal Protease Expedite de novo Protein Sequencing by Mass Spectrometry
Label-free protein sequencing is critically enabled by bottom-up, mass spectrometry-based proteomics workflows. Applications such as antibody sequencing or antigen discovery require de novo reconstruction of peptide and protein sequences. While trypsin has long served as the gold-standard protease i...
ORGANISM(S): Homo sapiens (Human) 
2026-03-17 | PXD063988 | Pride
Hta-proteases Hybrid fragmentation by eacid De novo antibody sequencing Bottom-up proteomics 
ProteomicsFrom Volcanos to the Bench: Advantages of Novel Hyperthermoacidic Archaeal Proteases for Proteomics Workflows
Here we introduce novel hyperthermoacidic archaeal proteases (HTA-Proteases) isolated from organisms that thrive in nearly boiling acidic volcanic springs and investigate their use for bottom-up proteomic experiments. We find that HTA-Proteases have novel cleavage specificities, show no autolysis, f...
ORGANISM(S): Bos taurus (Bovine) Mus musculus (Mouse) Homo sapiens (Human) Escherichia coli Sus scrofa domesticus (domestic pig) 
2023-10-18 | PXD041226 | Pride
Heat Hydrolysis Archaea Protease Ultrastable enzymes Hyperthermophile Sample preparation Acid Digestion 
UnknownDeep Coverage and Extended Sequence Reads Obtained with a Single Archaeal Protease Expedite <i>de novo</i> Protein Sequencing by Mass Spectrometry.
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S-EPMC12154832 | biostudies-literature
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