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Cells were lysed with TFA, and precipitated with cold acetone. The precipitates were transferred to E3filters and digestion was performed overnight with trypsin. The peptides were desalted with StageTips, and were analyzed on Eclipse Orbitrap with DIA mode. For MS1, the resolution was set to 120,00...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2024-02-27 | MSV000094179 | MassIVE
Mediator complex is an integrative hub for transcriptional regulation. Here we show that Mediator regulates alternative mRNA processing via its Med23 subunit. Combining tandem affinity purification and mass spectrometry, we identified a number of mRNA processing factors that bind to a soluble recomb...
ORGANISM(S): Homo sapiens 
Hemp is a Pb-tolerant and Pb-accumulating plant, and the study of its tolerance mechanisms could facilitate the breeding of hemp with enhanced Pb tolerance and accumulation. In the present study, we took advantage of SWATH (sequential window acquisition of all theoretical mass spectra) technology to...
ORGANISM(S): Eupatorium Cannabinum Iranian Hemp Witches'-broom Phytoplasma 
2019-05-04 | PXD013716 |
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