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ProteomicsProteome allocation strategies under nutrient-limiting conditions in the cyanobacterium Synechocystis
The response of the model cyanobacterium Synechocystis sp. PCC6803 towards light and carbon limitation was systematically probed. To this end, Synechocystis sp. PCC6803 was cultivated in a photo-bioreactor driven in turbidostat-mode. The turbidostat is a continuous cultivation that enabled cells to ...
ORGANISM(S): Synechocystis sp. PCC 6803 substr. GT-S 
2018-09-12 | PXD009582 | Pride
Shotgun proteomics Cyanobacteria Msgf+ Cellular economy Carbon limitation Openms Resource allocation Data-dependent acquisition Light limitation 
ProteomicsMetabolite interactions in the bacterial Calvin cycle and implications for flux regulation
Metabolite-level regulation of enzyme activity is important for microbes to cope with environmental shifts. Knowledge of such regulations can also guide strain engineering to improve industrial phenotypes. Recently developed chemoproteomics workflows allow for genome-wide detection of metabolite-pro...
ORGANISM(S): Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) Hydrogenophaga pseudoflava Synechocystis sp. (strain PCC 6803 / Kazusa) Bacteria Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) 
2023-08-23 | PXD044412 | Pride
Cupriavidus necator Cyanobacteria Prosit Synechococcus sp. pcc7942 Interaction proteomics Encyclopedia Hydrogenophaga pseudoflava Lip-smap Carbon fixation Synechocystis sp. pcc6803 Ralstonia eutropha 
ProteomicsThe fitness landscape of a Form II Rubisco in a photosynthetic bacterium guides engineering of oxygen tolerance
A pooled library of Synechocystis PCC6803 mutants expressing different RuBisCO variants were grown in turbidostats in different gas conditions and the proteome was analyzed by DIA LC/MS proteomics with a Q Exactive HF mass spectrometer after 0, 4 and 8 generations of growth
ORGANISM(S): Synechocystis sp. PCC 6803 
2026-07-16 | PXD071825 | Pride
Synechocystis Machine learning Cyanobacteria Rubisco Carbon fixation Enzyme engineering 
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