Human adrenal gland

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Technology Type

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Data-independent acquisition (1)

Instrument Platform

Illumina NovaSeq 6000 (18)
Illumina HiSeq 4000 (13)
NextSeq 500 (12)
Illumina HiSeq 2500 (11)
NextSeq 2000 (5)
PALM Robo LCM (3)
ScanArray 5000 [PerkinElmer] (3)
ScanArray Express HT [PerkinElmer] (3)
Scanning hardware (3)
Single-Cell Extraction: For enzymatic digestion, pronase (Septomyces argeus, 1 mg/mL) was applied for 25 minutes at room temperature for P0 to P8 datasets. Cells were dissociated and triturated into a single-cell suspension in ACSF supplemented with 1% fetal calf serum (FCS) and DNase (1 µL/10 mL). Trituration was performed using three glass Pasteur pipettes of decreasing diameters. For P30 datasets, enzymatic digestion and cell dissociation were carried out using the Worthington Papain Dissociation System, following the manufacturer’s instructions. Single-Nuclei Extraction: For nuclei isolation, the Hibernate™-E Medium was removed, and 500 µL of chilled 0.1X NP40 Lysis Buffer was added. Tissue was homogenized using a Pellet Pestle (15 strokes) and incubated on ice for 5 minutes. The suspension was pipette-mixed 10 times using a wide-bore pipette tip and incubated for an additional 10 minutes on ice. Following lysis, 500 µL of chilled wash buffer was added, and the mixture was pipette-mixed 5 times using a regular-bore pipette tip. The suspension was filtered through a 30 µm cell strainer into a 50 mL tube to remove debris. The filtered suspension was transferred to a 1.5 mL tube and centrifuged at 950 × g for 10 minutes at 4°C. The supernatant was carefully removed, and the nuclei pellet was retained for downstream analysis. (2)
Axon- GenePix4000B (2)
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Clariom D Human Transcriptome Arrays (Applied Biosystems, ThermoFisher) (1)
Thermal cycle, microcenrifuge, vortex mixer, pipettes (1)
Ionic purification system (Bionano Genomics) (1)
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Axon GenePix4000B (1)
Ion Chef™ System and Ion S5™ System or Ion GeneStudio™ S5 Systems. (1)
Not applicable (1)
Affymetrix GeneChip Scanner 3000 7G (1)

Publication Date

2010 (433)
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2019 (3)
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UNIPROT ID/AC

Q16850 (5)
Q86SK9 (5)
Q9BQG2 (1)
B3KXN9 (1)
Q53HM1 (1)
Q4FEB3 (1)
Q9HBF5 (1)
E9KL42 (1)
Q3I5F7 (1)
P33897 (1)
Q99884 (1)
A8MU28 (1)
Q8N130 (1)
P35219 (1)
Q16698 (1)
Q5FC00 (1)
P83881 (1)
B2RBR4 (1)
Q8WUM9 (1)
Q15274 (1)
Q8WWX8 (1)
Q9NX14 (1)
Q9Y345 (1)
Q16635 (1)
Q9NST1 (1)
Q86YT5 (1)
Q96N66 (1)
Q53H96 (1)
Q6ZWT7 (1)
Q92604 (1)

ENSEMBL ID

ENSG00000229353 (4)
ENSG00000170477 (4)
ENSG00000147251 (2)

CHEBI ID

CHEBI:3554 (1)
CHEBI:3338 (1)
CHEBI:3966 (1)
CHEBI:3312 (1)
CHEBI:3313 (1)
CHEBI:3628 (1)
CHEBI:3454 (1)
CHEBI:3544 (1)
CHEBI:4541 (1)
CHEBI:3322 (1)
CHEBI:3729 (1)
CHEBI:3420 (1)
CHEBI:3303 (1)
CHEBI:3513 (1)
CHEBI:3339 (1)
CHEBI:37632 (1)
CHEBI:58216 (1)
CHEBI:37636 (1)
CHEBI:37635 (1)
CHEBI:37634 (1)
CHEBI:58235 (1)
CHEBI:52288 (1)
CHEBI:3793 (1)
CHEBI:58414 (1)
CHEBI:58600 (1)
CHEBI:36986 (1)
CHEBI:58052 (1)
CHEBI:57658 (1)
CHEBI:57836 (1)
CHEBI:18402 (1)

Metabolite Name

7-methylguanosine (2)
2'-O-methyluridine (2)
Lactate (2)
Glutamate (2)
12,13-DiHOME (2)
Kynurenine (2)
Alanine (2)
Taurine (2)
Histidine (2)
Indolelactic acid (2)
PE-NMe (44:4) (1)
PE-NMe(42:4) (1)
Phosphatidylinositol (P-36:5) (1)
Ganglioside GM3 (36:2) (1)
Phosphatidic acid (P-36:3) (1)
Cardiolipin 78:13(2-) (1)
Cardiolipin 78:11(2-) (1)
CL(79:2) (1)
Phosphatidylethanolamine 40:3 zwitterion (1)
Phosphatidylethanolamine 36:5 zwitterion (1)
Phosphatidylethanolamine 32:1 zwitterion (1)
Phosphatidylethanolamine 42:2 (1)
PE-NMe(36:4) (1)
Phosphatidylglycerol 32:1(1-) (1)
CL(73:0) (1)
Phosphatidylserine 40:1(1-) (1)
Cardiolipin 78:14(2-) (1)
Phosphatidylinositol 42:6(1-) (1)
PG(O-34:0) (1)
Phosphatidylethanolamine 42:3 zwitterion (1)

First Public Date

2023 (9)
2024 (7)
2015 (6)
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2021 (5)
2025 (4)
2022 (3)
2014 (3)
2020 (2)
2019 (1)
2017 (1)
2016 (1)

Modelling Approach

Ordinary differential equation model (1)

Study status

Active, not recruiting (1)

Lab affiliation

Department of Peptide-based Immunotherapy, University and University Hospital Tuebingen, Germany (1)
Department of Immunology, Interfaculty Institute for Cell Biology, Eberhard Karls University Tübingen (1)
Laboratory of Cellular Structure and Function, Department of Anatomy; Institute of Biomedical Science, University of São Paulo, São Paulo,Brazil. (1)
Principal Investigator and Associate Director – VIB-UGent Center for Medical Biotechnology, Belgium Head of Department – UGent Department of Biomolecular Medicine, Belgium (1)
University of Zurich, Switzerland (1)
Proteomics Core Facility, Institute of Immunology, Rikshospitalet Oslo Norway (1)
Institute of Biomedical Sciences, Department of Anatomy, University of São Paulo,São Paulo, Brazil, Av. Prof. Lineu Prestes, 2415, Butantã, São Paulo, SP, 05508-000. (1)
Chair of Proteomics and Bioanalytics, Technical University of Munich, Freising, Germany Bavarian Center for Biomolecular Mass Spectrometry, Freising, Germany (1)
Institute of Biomedical Sciences, Department of Anatomy, University of São Paulo, São Paulo, Brazil, Av. Prof. Lineu Prestes, 2415, Butantã, São Paulo, SP, 05508-000 (1)
1. Paris-Cité university, Cochin institute CNRS UMR8104, Inserm U1016, 24 rue du faubourg Saint Jacques 75014 Paris, France 2. Department of Endocrinology and National Reference Center for Rare Adrenal Disorders, Cochin hospital, Assistance Publique Hôpitaux de Paris, 24 rue du faubourg Saint Jacques 75014 Paris, France (1)
Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Zurich, 8093, Switzerland (1)
McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 USA (1)
Proteomics Core Facility, University of Gothenburg, Sweden (1)
Chair of Proteomics and Bioanalytics, Technische Universität München, Germany (1)
Wellcome Trust Sanger Institute (1)

Keyword

Ipilimumab (1)
Nivolumab (1)

IsPrivate

False (2)

Model Flag

Non Kinetic (1)

Study type

Transcription profiling by array (1450)
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Animal - High-throughput sequencing (3)
Ribo-seq (3)
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Cell line - One-color microarray (1)
Cell line - High-throughput sequencing (1)
MicroRNA profiling by high throughput sequencing (1)
Spatial transcriptomics by high-throughput sequencing (1)
ATAC-seq (1)
Single nucleus RNA sequencing (1)
Genotyping by high throughput sequencing (1)
Interventional (1)

Release Date

2010 (440)
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2013 (196)
2009 (117)
2008 (81)
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2007 (62)
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2003 (2)
2000 (2)
2004 (1)
2002 (1)
1998 (1)

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Single-nucleus and spatial transcriptome reveal adrenal homeostasis in normal and tumoural adrenal glands

The human adrenal glands are highly dynamic endocrine organs that are involved in the secretion of various hormones such as steroids and catecholamines. Here we present a single-nuclei and spatial transcriptomic analysis of healthy adult human adrenal glands to provide a complete adrenal gland atlas...

ORGANISM(S): Homo sapiens

2023-01-01 | E-MTAB-12129 | biostudies-arrayexpress

Transcription profiling of mouse adrenal gland samples at multiple time points after release into DD to characterise rhythmic gene expression in the adrenal gland

This study was performed to characterize the rhythmic transcriptome of the murine adrenal gland with a focus on the corticosterone synthesis pathway. Experiment Overall Design: LD entrained male animals were released into DD and from the second day on whole adrenals were prepared from 12 time points...

ORGANISM(S): Mus musculus

2007-12-12 | E-GEOD-4238 | biostudies-arrayexpress

Adrenal gland gene expression from adult (12-week old) hypertensive BPH and adult (12-week old) hypotensive BPL mice

The objective of the experiment is to determine the genes differentially expressed in the adrenal gland of adult (12-week) BPH and BPL mice. Adrenal glands from adult (12-week) hypertensive BPH mice (n=3) and adult (12-week) hypotensive BPL mice (n=3).

ORGANISM(S): Mus musculus

2010-06-16 | E-GEOD-19342 | biostudies-arrayexpress

Decoding the human adrenal cortex matrisome: the composition and regulation of ECM proteins of the outer and inner microenvironment.

The extracellular matrix (ECM) comprises macromolecules that shape a complex three-dimensional network. Filling the intercellular space and playing a crucial role in the structure and function of tissues, ECM regulates essential cellular processes such as adhesion, differentiation, and cell signalin...

ORGANISM(S): Homo sapiens (Human)

2024-09-11 | PXD051801 | Pride

Adrenal gland gene expression from chromogranin A knockout mice (Mahapatra et al. 2005) vs. wild-type mice

The objective of the experiment is to determine the genes differentially expressed in the adrenal gland of the chromogranin A knockout mouse (Mahapatra et al., 2005). Adrenal glands from chromogranin A knockout mice (n=3) and wild-type control mice (n=3).

ORGANISM(S): Mus musculus

2010-03-15 | E-GEOD-18304 | biostudies-arrayexpress

Microarray study of human adrenal zona glomerulosa (ZG), zona fasciculata (ZF) and aldosterone-producing adenomas (APA)

Learn about the transcriptome profiling of zona glomerulosa (ZG), zona fasciculata (ZF) and aldosterone-producing adenomas (APA) in human adrenals 21 pairs of zona fasciculata (ZF) and zona glomerulosa (ZG), and 14 paired aldosterone-producing adenomas (APAs) from 14 Conn’s syndrome patients and 7 p...

ORGANISM(S): Homo sapiens

2015-09-15 | E-GEOD-64957 | biostudies-arrayexpress

Genome wide analysis of Adrenal Gland tissue from Gata6 conditional knockout versus Control Adrenal tissue

Transcription factor GATA6 is expressed in the fetal and adult adrenal cortex and has been implicated in steroidogenesis. To characterize the role of GATA6 in adrenocortical development and function, we generated mice in which Gata6 was conditionally deleted using Cre-LoxP recombination with Sf1-cre...

ORGANISM(S): Mus musculus

2013-06-06 | E-GEOD-40398 | biostudies-arrayexpress

PACAP dependency of genes regulated in adrenal gland

We investigated the effects of PACAP knock out on the transcriptosome profile of adrenal gland. A 35,546-clone mouse cDNA microarray was used to investigate transcriptional changes Keywords: transcript identification design In the hybridization method employed, equal amounts (2-5 ug/15.5 ul) of tota...

ORGANISM(S): Mus musculus

2010-05-26 | E-GEOD-7405 | biostudies-arrayexpress

Proteomic analysis of the extracellular matrix in the porcine adrenal cortex

The extracellular matrix (ECM) is a complex network of proteins that provides structural support and influences tissue boundaries, biomechanical properties, and cell polarity. This study analyzed the ECM profile of the porcine adrenal cortex's outer (OF = capsule + subcapsular + zona glomerulosa cel...

ORGANISM(S): Sus scrofa domesticus (domestic pig)

2025-07-14 | PXD055486 | Pride

Adrenal cortex Extracellular matrix Adrenal gland Porcine

RNA-seq of human fetal adrenal gland and control tissues

The dataset contains RNA-seq for 32 human fetal adrenal gland samples between 7 weeks post-conception (wpc) and 11.5 wpc and 14 control samples from different tissues across the same time frame. The study is designed with 8 fetal adrenal samples (4 of 46,XY; 4 of 46,XX) at 7wpc, 8wpc, 9wpc, 11.5wpc

ORGANISM(S): Homo sapiens

2023-02-01 | E-MTAB-12492 | biostudies-arrayexpress

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