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ProteomicsAtLEGbeta / gamma protease specificity and putative substrate characterization by HUNTER
Arabidopsis thaliana Legumain (aka VPE) beta / gamma were characterized for their cleavage specificity and putative substrates were identified by the N-termini enrichment method HUNTER.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-07-29 | PXD019276 | Pride
Arabidopsis thaliana legumain vpe beta gamma protease hunter substrates 
ProteomicsOptimization of HUNTER N-termini profiling using rat brain proteome extracts
Optimization hydrophobic tagging reaction time (30 min to 2h) and evaluation of the HUNTER protocol performance with different amounts (25 ug to 400 ug) of purified rat brain proteome as starting material.
ORGANISM(S): Rattus norvegicus (Rat) 
2019-09-03 | PXD012844 | Pride
Wistar rat hunter n-termini 
TranscriptomicsVitamin d receptor-mediated stromal reprogramming suppresses pancreatitis and enhances pancreatic cancer therapy
The poor clinical outcome in pancreatic ductal adenocarcinoma (PDA) has been attributed to intrinsic resistance to chemotherapy and a growth-permissive tumor microenvironment. Quiescent pancreatic stellate cells (PSCs) are neuroendocrine, nestin-positive, lipid-accumulating cells whose homologues i...
ORGANISM(S): Mus musculus 
2014-10-03 | E-GEOD-43770 | biostudies-arrayexpress
GenomicsThe DAXX co-repressor is directly recruited to active regulatory elements genome-wide to regulate autophagy programs in a model of human prostate cancer (ChIP-seq)
This study was aimed at understanding the genome-wide binding and regulatory role of the DAXX transcriptional repressor, recently implicated in PCa. ChIP-Seq analysis of genome-wide distribution of DAXX in PC3 cells revealed over 59,000 DAXX binding sites, found at regulatory enhancers and promoters...
ORGANISM(S): Homo sapiens 
2015-06-24 | E-GEOD-68647 | biostudies-arrayexpress
ProteomicsOptimization of HUNTER N-termini profiling using Arabidopsis thaliana Col-8 wt leaf proteome extracts
Optimization hydrophobic tagging reagent (hexadecanal or undecanal), reaction time (30 min to 16h) and evaluation of the HUNTER protocol performance with different amounts (25 ug to 400 ug) of purified Arabidopsis thaliana Col-8 wt leaf proteome as starting material.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-09-03 | PXD012821 | Pride
Hunter n-termini arabidopsis thaliana col-8 
ProteomicsHUNTER N-termini profiling of Arabidopsis thaliana leaves using different proteases
Comparison of protein N-termini in Arabidopsis thaliana leaves by the HUNTER method; the corresponding digestion was performed with legumain, GluC and trypsin.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2020-01-20 | PXD014697 | Pride
Arabidopsis hunter n-termini profiling 
TranscriptomicsThe DAXX co-repressor is directly recruited to active regulatory elements genome-wide to regulate autophagy programs in a model of human prostate cancer (RNA-seq)
This study was aimed at understanding the genome-wide binding and regulatory role of the DAXX transcriptional repressor, recently implicated in PCa. ChIP-Seq analysis of genome-wide distribution of DAXX in PC3 cells revealed over 59,000 DAXX binding sites, found at regulatory enhancers and promoters...
ORGANISM(S): Homo sapiens 
2015-06-24 | E-GEOD-68645 | biostudies-arrayexpress
UnknownA prospective study of plasma vitamin D metabolites, vitamin D receptor polymorphisms, and prostate cancer.
Not available
S-EPMC1831738 | biostudies-literature
UnknownBurkholderia pseudomallei d-alanine-d-alanine ligase; detailed characterisation and assessment of a potential antibiotic drug target.
Not available
S-EPMC6899670 | biostudies-literature
ProteomicsHUNTER N-termini profiling of Arabidopsis thaliana wt and vpe0 mutants
Comparison of protein termini in Arabidopsis thaliana vpe0 quadruple mutant and wildtype seedlings shortly after germination to identify differential processed proteins.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-09-03 | PXD012804 | Pride
Arabidopsis vpe0 termini degradomics hunter 
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