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ProteomicsDeep Coverage and Extended Sequence Reads Obtained with a Single Archaeal Protease Expedite de novo Protein Sequencing by Mass Spectrometry
Label-free protein sequencing is critically enabled by bottom-up, mass spectrometry-based proteomics workflows. Applications such as antibody sequencing or antigen discovery require de novo reconstruction of peptide and protein sequences. While trypsin has long served as the gold-standard protease i...
ORGANISM(S): Homo sapiens (Human) 
2026-03-17 | PXD063988 | Pride
Hta-proteases Hybrid fragmentation by eacid De novo antibody sequencing Bottom-up proteomics 
ProteomicsSpectral quality guided optimization of hybrid EAciD fragmentation on the ZenoTOF 7600 system
Systematically optimized N-glycoproteomics method combining EAD and CID on the ZenoTOF 7600. Several dynamic CID parameters were optimized, next EAD was added using different kinetic energy values. Optimization was performed on glycopeptide enriched human plasma. After optimization, the method was ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-07-15 | MSV000098519 | MassIVE
UnknownDeep Coverage and Extended Sequence Reads Obtained with a Single Archaeal Protease Expedite <i>de novo</i> Protein Sequencing by Mass Spectrometry.
Not available
S-EPMC12154832 | biostudies-literature
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