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A challenge for shotgun proteomics is the identification of low abundance proteins, which is always hampered owing to the extreme complexity of protein digests and highly dynamic concentration range of proteins. To reduce the complexity of the peptide mixture, we developed a novel method to selectiv...
ORGANISM(S): Homo Sapiens 
Here we describe a peptide-centric approach (OXIPEP), where carbonylated residues within proteins are derivatized with biotin-hydrazide, enriched and identified by mass spectrometry. The strength of the method lies in an improved protocol for elution of biotinylated peptides from monomeric avidin r...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2018-10-18 | PXD002966 | Pride
Isoniazid induced varying degrees of hepatic steatosis in an inbred strain Mouse Diversity Panel (MDP) study. RNA was isolated from all animals for analysis of gene expression changes in the liver. The objective of this study was to identify gene expression changes that drive isoniazid-induced steat...
ORGANISM(S): Mus musculus 
Study of a hydrazide degrading mechanism by Miacrobacterium sp.
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