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MetabolomicsAn LCMS-based untargeted metabolomics protocol for cochlear perilymph: highlighting metabolic effects of hydrogen gas on the inner ear of noise exposed Guinea pigs

INTRODUCTION: Noise-induced hearing loss (NIHL) is an increasing problem in society and accounts for a third of all cases of acquired hearing loss. NIHL is caused by formation of reactive oxygen species (ROS) in the cochlea causing oxidative stress. Hydrogen gas (H2) ...

2021-10-06 | MTBLS2418 | MetaboLights
MetabolomicsIdentifying a tryptophan derivative in hydrogen peroxide-treated cell culture medium
Not available
ST002174 | MetabolomicsWorkbench
TranscriptomicsGene expression profiling in mouse livers after administration of both hydrogen-rich water and hydrogen gas
Molecular hydrogen is a hopeful agent for oxidative stress-related and/or inflammatory disorders. However, the molecular mechanism for these therapeutic effects of hydrogen still remains to be fully elucidated. We examined whether molecular hydrogen alters gene expression levels in normal mouse live...
ORGANISM(S): Mus musculus 
2014-08-01 | E-GEOD-48826 | biostudies-arrayexpress
TranscriptomicsRedirection of metabolism for biological hydrogen production
Photosynthetic microbes can produce the clean-burning fuel hydrogen using one of natureâ??s most plentiful resources, sunlight 1,2. Anoxygenic photosynthetic bacteria generate hydrogen and ammonia during a process known as biological nitrogen fixation. This reaction is catalyzed by the enzyme nitrog...
ORGANISM(S): Rhodopseudomonas palustris 
2010-06-10 | E-GEOD-5194 | biostudies-arrayexpress
TranscriptomicsInjury-dependent hydrogen peroxide oxidation of IKK-alpha regulates keratinocyte migration through induction of EGF
Hydrogen peroxide is known to promote skin keratinocyte migration, although the mechanism of action is unclear. In an attempt to identify signaling pathways regulated by hydrogen peroxide in the skin, 3 day post fertilized (dpf) zebrafish larvae (nacre strain) were treated with 3mM hydrogen peroxide...
ORGANISM(S): Danio rerio 
2016-02-22 | E-GEOD-75728 | biostudies-arrayexpress
TranscriptomicsHydrogen sulfide stimulates Mycobacterium tuberculosis respiration and growth to exacerbate TB disease
The aim of this experiment was to determine how exposure of Hydrogen sulfide impacts gene expression in Mycobacterium tuberculosis. RNA was isolated from actively growing mycobacterial cells (0.6-0.8 OD600) using Trizol according to established protocols (27). Briefly, cells were exposed to 25 µM G...
ORGANISM(S): Mycobacterium tuberculosis H37Rv 
2020-01-04 | E-MTAB-7421 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of E. coli TG1/pBS(Kan)Synhox and pBS(Kan) ability to produce hydrogen
E. coli TG1 with pBS(Kan)Synhox can produce more hydrogen than TG1/pBS(Kan). To reveal the difference of metabolic activity (gene expression) between these strains in producing hydrogen, the differential gene expression analyses were performed. All samples cultured in complex medium with fructose ...
ORGANISM(S): Escherichia coli 
2008-06-14 | E-GEOD-5905 | biostudies-arrayexpress
TranscriptomicsIdentification of Gene Products Involved in the Oxidative Stress Response of Moraxella catarrhalis: Evaluation of OxyR dependent Genes
Series of DNA microarrays (4) comparing the M. catarrhalis strain 035E and M. catarrhalis oxyR mutant response to 50 mM hydrogen peroxide. Wild-type cells and oxyR mutant cells were exposed to 50 mM hydrogen peroxide for 15 minutes. RNA was extracted and DNA microarray analysis performed. 4 biologic...
ORGANISM(S): Moraxella catarrhalis 
2010-12-07 | E-GEOD-24351 | biostudies-arrayexpress
TranscriptomicsDe Novo Transcriptomic Analysis of Hydrogen Production in the Green Alga Chlamydomonas moewusii through RNA-Seq
Background: Microalgae can make a significant contribution towards meeting global renewable energy needs in both lipid-based liquid biofuel and hydrogen biofuel. The development of energy-related products and chemicals from algae could be accelerated with improvements in systems biology tools, and r...
ORGANISM(S): Chlamydomonas moewusii 
2013-09-04 | E-GEOD-46225 | biostudies-arrayexpress
TranscriptomicsHydrogen vs Lactate as electron donor in Sulfate reduction
In order to obtain a global view of energy metabolism pathways of the sulfate-reducer Desulfovibrio vulgaris Hildenborough and the proteins involved therein whole-genome microarrays were used to compare the transcriptional response of cells grown with hydrogen/sulfate, pyruvate/sulfate, lactate/thio...
ORGANISM(S): Desulfovibrio vulgaris subsp. vulgaris str. Hildenborough 
2008-05-31 | E-GEOD-8037 | biostudies-arrayexpress
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