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Deletion of a hydrogenase maturation factor (HydG) knocks out the assembly of mature, functioning hydrogenases to divert metabolic flux away from hydrogen production and towards ethanol. Other system-wide affects assessed.
ORGANISM(S): Clostridium thermocellum (strain DSM 1313 / LMG 6656 / LQ8) 
2016-11-03 | PXD000777 | Pride
Background: Microalgae can make a significant contribution towards meeting global renewable energy needs in both lipid-based liquid biofuel and hydrogen biofuel. The development of energy-related products and chemicals from algae could be accelerated with improvements in systems biology tools, and r...
ORGANISM(S): Chlamydomonas moewusii 
Aerobic methanotrophic bacteria can use methane as their sole energy source. The discovery of ‘Ca. Methylacidiphilum fumariolicum’ strain SolV and other verrucomicrobial methanotrophs has revealed that the ability of bacteria to oxidize CH4 is much more diverse than has previously been assumed in te...
ORGANISM(S): Methylacidiphilum fumariolicum 
The unicellular cyanobacterium Synechocystis sp. PCC 6803 is a model system for studying biochemistry, genetics and molecular biology of photobiological processes. Despite its importance in basic and applied research, the genome-wide picture of transcriptional regulation in this bacterium is limited...
ORGANISM(S): Synechocystis sp. PCC 6803 
Clostridium thermocellum is a Gram-positive, anaerobic, thermophilic bacterium that ferments cellulose into ethanol. It is a candidate industrial consolidated bioprocess (CBP) biocatalyst for lignocellulosic bioethanol production. However, C. thermocellum is relatively sensitive to ethanol compared ...
ORGANISM(S): Clostridium thermocellum ATCC 27405 
The sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough possesses four periplasmic hydrogenases to facilitate the oxidation of molecular hydrogen. These include an [Fe], a [NiFeSe] and two [NiFe] hydrogenases encoded by the hyd, hys, hyn1 and hyn2 genes, respectively. In order to underst...
ORGANISM(S): Desulfovibrio vulgaris subsp. vulgaris str. Hildenborough 
To study mixotrophy, it is desirable to have an organism capable of growth in the presence and absence of both organic and inorganic carbon sources, as well as organic and inorganic energy sources. Metallosphaera sedula is an extremely thermoacidophilic archaeon which has been shown to grow in the p...
ORGANISM(S): Metallosphaera sedula 
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