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ProteomicsHydroxyl Radical and Trifluoromethyl Carbohydrate Footprinting for Probing Protein Binding Components of Oligosaccharides
The NAG3 was modified by hydroxyl radicals and CF3 radicals and determined the end residues where these modifications occur.
ORGANISM(S): Homo sapiens (Human) 
2025-09-29 | PXD066426 | Pride
Cf3 radical Rcf Hydroxyl radical Oxidation Nag3 Modification 
ProteomicsDimethylthiourea as a Quencher in Hydroxyl Radical Protein Footprinting Experiments
Hydroxyl radical protein footprinting (HRPF) is a mass spectrometry (MS)-based method for studying protein structures, interactions, conformations, and folding. We evaluated dimethylthiourea (DMTU) as a replacement for catalase in quenching HRPF reactions. We observed that the DMTU is highly effecti...
ORGANISM(S): Escherichia coli 
2025-09-12 | PXD046435 | Pride
Hrpf Dimethylthiourea Quencher 
TranscriptomicsMultiplexed RNA structure characterization with selective 2'-hydroxyl acylation analyzed by primer extension sequencing (SHAPE-Seq)
New regulatory roles continue to emerge for both natural and engineered noncoding RNAs, many of which have specific secondary and tertiary structures essential to their function. Thus there is a growing need to develop technologies that enable rapid characterization of structural features within com...
ORGANISM(S): Bacillus subtilis 
2011-08-26 | E-GEOD-31573 | biostudies-arrayexpress
GenomicsMorc2a variants cause hydroxyl radical-mediated neuropathy and are rescued by GHKL ATPase gene therapy
Morc2a variants cause hydroxyl radical-mediated neuropathy and are rescued by GHKL ATPase gene therapy
PRJNA988036 | ENA
Proteomics10-HDA reduces the hydroxyl free radical-induced damage to vascular smooth muscle cells by rescuing protein and energy metabolisms
10-hydroxydec-2-enoic acid (10-HDA), a natural agent from royal jelly, could fight against cell and tissue damage, yet the underlying mechanisms are still unexplored. Here, we found the scavenging rate of hydroxyl free radical (•OH) by 2% (g/mL) 10-HDA was more than 20%, which was achieved by a mult...
ORGANISM(S): Mus Musculus 
2021-11-22 | PXD029873 |
TranscriptomicsMorc2a variants cause hydroxyl radical-mediated neuropathy and are rescued by GHKL ATPase gene therapy
Mutations in the Microrchidia CW-Type Zinc Finger 2 (MORC2) GHKL ATPase module cause Charcot Marie Tooth type 2Z or a broad range of neuropathy, but etiology and therapeutic strategy are not fully defined. Previously, we reported that the Morc2a p.S87L mouse model led to neuropathy and muscular dysf...
ORGANISM(S): Mus musculus 
2024-02-06 | GSE235918 | GEO
TranscriptomicsCapture and sequence analysis of RNAs with 5'-hydroxyl termini in S. cerevisiae
Data from 5OH-seq experiments to map RNAs with 5'-hydroxyl termini in S. cerevisiae 17 samples of yeast RNA analyzed by 5OH-seq. 2 Replicates: +/- Xrn1,+/- Tm, +/- SAP. 1 replicate: pre-fragmented RNA
ORGANISM(S): Saccharomyces cerevisiae 
2015-04-18 | E-GEOD-61527 | biostudies-arrayexpress
UnknownHydroxyl Radical Dosimetry for High Flux Hydroxyl Radical Protein Footprinting Applications Using a Simple Optical Detection Method.
Not available
S-EPMC4631671 | biostudies-literature
ProteomicsRadical footprinting in mammalian whole blood
Hydroxyl Radical Protein Footprinting (HRPF) is a powerful tool to probe protein higher-order structure, as well as protein-protein and protein-carbohydrate interactions. It is mostly performed in vitro, but recent advances have extended its use to live cells, nematodes, and 3D cultures. However, ap...
ORGANISM(S): Mus musculus (Mouse) 
2026-01-13 | PXD064571 | Pride
Hrpf Targeted analysis Mouse blood Diabetes mellitus 
UnknownIntrinsic Buffer Hydroxyl Radical Dosimetry Using Tris(hydroxymethyl)aminomethane.
Not available
S-EPMC7650270 | biostudies-literature
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