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ProteomicsProximity proteomics of T-tubules using miniTurbo-fused LUBEL
To identify candidate genes unbiasedly, we employed biotinylation-based proximity proteomics of T-tubules in vivo. We generated a transgenic line carrying an LUBEL construct fused with miniTurbo (mnTb). We expressed either LUBEL-mnTb or mnTb alone in muscles, and larvae were reared in the presence o...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-11-22 | PXD062634 | JPOST Repository
ProteomicsCo-IP and MS analysis of LacZ or 3xHA:LUBEL-N
HA-LUBEL 1-872 or LacZ (control) was expressed under the control of Mef2-GAL4, and the progenies were raised at 25°C. The 3IL carcass fillet lysates were subjected to anti-HA immunoprecipitation. To identify potential binding partners, we performed immunoprecipitation-mass spectrometry (IP-MS) of LU...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-11-22 | PXD062558 | JPOST Repository
ProteomicsProximity proteomics of T-tubules
To identify candidate genes unbiasedly, we employed biotinylation-based proximity proteomics of T-tubules in vivo. We generated a transgenic line carrying an Amph construct fused with miniTurbo (mnTb). We expressed either Amph-mnTb or mnTb alone in muscles, and larvae were reared in the presence or ...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-11-22 | PXD062543 | JPOST Repository
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