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TranscriptomicsCapTrap-Seq: A platform-agnostic and quantitative approach for high-fidelity full-length RNA transcript sequencing
Here we describe CapTrap-Seq, an experimental workflow designed to address the problem of reduced transcript end detection by long-read RNA sequencing methods, especially at the 5' ends. We apply CapTrap-Seq to profile transcriptomes of the human heart and brain and we compared the obtained results ...
ORGANISM(S): Mus musculus 
2023-12-20 | E-MTAB-13063 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse eGFP/CD3-double positive splenocytes to identify imprinted genes expressed in adult CD3+ splenocytes
Identification of imprinted genes expressed in adult CD3+ splenocytes Experiment Overall Design: For analysis of imprinted gene expression of uniparental-derived cells in adult recipients we chose eGFP/CD3-double positive splenocytes as a defined cell type, and first identified imprinted genes that ...
ORGANISM(S): Mus musculus 
2008-06-15 | E-GEOD-6757 | biostudies-arrayexpress
TranscriptomicsStage-specific control of early B cell development by the transcription factor Ikaros
Ikaros is an essential regulator of lymphopoiesis. Here, we studied the B-cell-specific function of Ikaros by conditional Ikzf1 inactivation in pro-B cells. B-cell development was arrested at an aberrant ‘pro-B’ cell stage characterized by increased cell adhesion and loss of pre-B cell receptor sign...
ORGANISM(S): Mus musculus 
2014-02-09 | E-GEOD-53595 | biostudies-arrayexpress
TranscriptomicsCharacterization of cyclobutane pyrimidine dimer-dependent modulation of gene expression in human keratinocytes following UVB exposure
To address CPD-dependent UVB activities, a model system was established in which transfection of keratinocytes with pseudouridine-modified mRNA (M-NM-(-mRNA) encoding CPD-photolyase resulted in 90% reduction of CPDs within 6 and 24 hours after UVB exposure. Microarray analysis of this model system d...
ORGANISM(S): Homo sapiens 
2015-01-16 | E-GEOD-65034 | biostudies-arrayexpress
UnknownExcessive EP4 Signaling in Smooth Muscle Cells Induces Abdominal Aortic Aneurysm by Amplifying Inflammation.
Not available
S-EPMC7253191 | biostudies-literature
TranscriptomicsGene expression of human skin fibroblast cells derived from hypospadias patients in response to low-dose bisphenol A exposure
To better understand the molecular basis of the reproductive health effects of bisphenol A (BPA) on humans, a genome-wide screening was applied to identify novel targets of low-dose bisphenol A exposure in huamn skin fibroblast cells (hSFCs) derived from hypospadias patient children. Three hSFCs wer...
ORGANISM(S): Homo sapiens 
2012-01-13 | E-GEOD-35034 | biostudies-arrayexpress
TranscriptomicsModulation of primary cell function of host Pseudomonas bacteria by conjugative plasmid pCAR1
Impacts of plasmid carriage on its host cell were comprehensively analyzed using conjugative plasmid pCAR1 in the three different kinds of hosts, Pseudomonas putida KT2440, P. aeruginosa PAO1, and P. fluorescens Pf0-1. Various analyses of the host phenotype showed that pCAR1 carriage reduced host fi...
ORGANISM(S): Pseudomonas putida 
2012-12-25 | E-GEOD-39636 | biostudies-arrayexpress
TranscriptomicsAlterations of RNA maps of IncP-7 plasmid pCAR1 in various Pseudomonas bacteria.
RNA mapping on pCAR1 carried by KT2440, CA10, PAO1, Pf0-1, HS01, and JCM 2778 6 strains/biological entities were compared
ORGANISM(S): Pseudomonas aeruginosa 
2011-10-10 | E-GEOD-28568 | biostudies-arrayexpress
GenomicsFlexible chromatin loops in the VH gene region of the Igh locus facilitate the generation of a diverse antibody repertoire
The immunoglobulin heavy-chain (Igh) locus undergoes large-scale contraction in pro-B cells, which facilitates VH-DJH recombination by juxtaposing distal VH genes next to the DJH- rearranged gene segment in the proximal Igh domain. By high-resolution mapping of long-range interactions, we now demons...
ORGANISM(S): Mus musculus 
2013-09-30 | E-GEOD-43008 | biostudies-arrayexpress
ProteomicsSecretome of rat smooth muscle cells with stretch
The extracellular matrix (ECM) is fundamental to cellular and tissue structural integrity and provides mechanical cues to initiate diverse biological functions1. The quality and quantity of ECM determines tissue stiffness and controls gene expression, cell fate, and cell cycle progression in various...
ORGANISM(S): Rattus Rattus (black Rat) 
2020-03-18 | PXD013915 | JPOST Repository
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