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ProteomicsSite-specific ubiquitination of the E3 ligase HOIP regulates apoptosis and immune signaling
HOIP, the catalytic component of the Linear Ubiquitin chain Assembly Complex (LUBAC), is a critical regulator of inflammation. However, how HOIP itself is regulated to control inflammatory responses is unclear. Here, we discover that site-specific ubiquitination of K784 within HOIP promotes Tumour N...
ORGANISM(S): Homo sapiens (Human) 
2020-08-31 | PXD021217 | Pride
Site-specific ubiquitination of the e3 ligase hoip regulates apoptosis and immune signaling 
ProteomicsThe linear ubiquitin chain assembly complex LUBAC generates heterotypic ubiquitin chains
The linear ubiquitin chain assembly complex (LUBAC) is the only known ubiquitin ligase that generates linear/Met1-linked ubiquitin chains. One of the LUBAC components, HOIL-1L, was recently shown to catalyse oxyester bond formation between the C-terminus of ubiquitin and some substrates. However, ox...
ORGANISM(S): Homo sapiens (Human) 
2021-06-28 | PXD019771 | Pride
E3 ubiquitin ligase/ ester-bond linkage/ hoil-1l/ rbr/ ubiquitin 
ProteomicsProximity proteomics of T-tubules
To identify candidate genes unbiasedly, we employed biotinylation-based proximity proteomics of T-tubules in vivo. We generated a transgenic line carrying an Amph construct fused with miniTurbo (mnTb). We expressed either Amph-mnTb or mnTb alone in muscles, and larvae were reared in the presence or ...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-11-22 | PXD062543 | JPOST Repository
ProteomicsProximity proteomics of T-tubules using miniTurbo-fused LUBEL
To identify candidate genes unbiasedly, we employed biotinylation-based proximity proteomics of T-tubules in vivo. We generated a transgenic line carrying an LUBEL construct fused with miniTurbo (mnTb). We expressed either LUBEL-mnTb or mnTb alone in muscles, and larvae were reared in the presence o...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-11-22 | PXD062634 | JPOST Repository
ProteomicsCo-IP and MS analysis of LacZ or 3xHA:LUBEL-N
HA-LUBEL 1-872 or LacZ (control) was expressed under the control of Mef2-GAL4, and the progenies were raised at 25°C. The 3IL carcass fillet lysates were subjected to anti-HA immunoprecipitation. To identify potential binding partners, we performed immunoprecipitation-mass spectrometry (IP-MS) of LU...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2025-11-22 | PXD062558 | JPOST Repository
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