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MetabolomicsGolgi-IP, a tool for multimodal analysis of Golgi molecular content
The Golgi is a membrane-bound organelle that is essential for protein and lipid biosynthesis. It represents a central trafficking hub that sorts proteins and lipids to various destinations or for secretion from the cell. The Golgi has emerged as a docking platform for cellular signaling pathways inc...
2023-06-27 | MTBLS6511 | MetaboLights
ProteomicsDiscovery of Klf2 Interactors in Mouse Embryonic Stem Cells by Immunoprecipitation-Mass Spectrometry Utilizing Exogenously Expressed Bait
Krüppel-like factor 2 (Klf2) is a DNA-binding transcription factor that regulates embryonic stem cell-specific gene expression. Transcription cofactors such as p300 acetyltransferases and Erk kinases interact with Klf2, providing an additional layer of transcription regulation in embryonic stem cell...
ORGANISM(S): Mus Musculus (mouse) 
2021-09-20 | PXD022123 | JPOST Repository
GenomicsProtocol for massively parallel RNA assay coupled with immunoprecipitation (MPRNA-IP)
Protocol for massively parallel RNA assay coupled with immunoprecipitation (MPRNA-IP)
PRJNA1395057 | ENA
Proteomicsimmunoprecipitation-mass spectrometry (IP-MS) of FDR-1
immunoprecipitation-mass spectrometry (IP-MS)of FDR-1
ORGANISM(S): Caenorhabditis elegans 
2025-08-07 | PXD066847 | Pride
Fdr-1 ip-ms 
GenomicsArgonaute2 (Ago2) immunoprecipitation (IP) assay using miR-130b overexpressing A549 cells.
Argonaute2 (Ago2) immunoprecipitation (IP) assay using miR-130b overexpressing A549 cells.
PRJNA485051 | ENA
OtherAssessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.
Data from ProteomeXchange, PXD ID: PXD002534. File: GR_130129_20_OT3_NT_L3MBTL2_FAB_31_G11_2_HEK293.mzXML. Published as part of Nat Methods. 2015 Jun 29 . From the Abstract: {{i}} ... We describe a mass spectrometry-based standard operating procedure for scoring immunoprecipitation antibody quality...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
GPM32320016573 | GPMDB
Resource Reanalysis 
TranscriptomicsNext Generation Sequencing Facilitates Quantitative Analysis of Argonaute 2 (Ago2)-immunoprecipitation (IP) after miR-195 or miR-497 overexpression in HepG2
To explore functionally crucial tumor-suppressive (TS)-miRNAs in hepatocellular carcinoma (HCC), we performed integrative function- and expression-based screenings of TS-miRNAs in six HCC cell lines. The screenings identified seven miRNAs, which showed growth-suppressive activities through the over...
ORGANISM(S): Homo sapiens 
2013-05-31 | E-GEOD-41074 | biostudies-arrayexpress
TranscriptomicsIdentification of miRNA targets in breast cancer cells (Ago2-IP)
miRNAs regulate mRNA stability and translation through the action of the RNAi-induced silencing complex. In this study, we systematically identified endogenous miRNA target genes by using AGO2 immunoprecipitation (AGO2-IP) and microarray analyses in two breast cancer cell lines, MCF7 and MDA-MB-231,...
ORGANISM(S): Homo sapiens 
2013-10-21 | E-GEOD-48158 | biostudies-arrayexpress
ProteomicsAkt binding proteins - We try to identify Akt interaction proteins and Akt modifications. For mass spectrometry analysis, immunoprecipitation (IP) was performed with the whole cell lysates in different treated cells.
We try to identify Akt interaction proteins and Akt modifications. For mass spectrometry analysis, immunoprecipitation (IP) was performed with the whole cell lysates in different treated cells.
ORGANISM(S): Homo sapiens (Human) 
2018-12-11 | PXD011966 | Pride
Akt 
TranscriptomicsIdentification of the mRNAs interacting with the Nup59 nucleoporin using RNA-IP and microarrays in budding yeast
To specifically explore such mRNA localization events, we immunopurified the entire Nuclear Pore Complex scaffold using Nup59 as a bait and systematically analyzed all co-isolating mRNAs using Agilent microarrays in the budding yeast Saccharomyces cerevisiae. A mock IP was performed as a control exp...
ORGANISM(S): Saccharomyces cerevisiae 
2021-03-31 | E-MTAB-9521 | biostudies-arrayexpress
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