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TranscriptomicsIdentification of genes directly regulated by the oncogene ZNF217 using ChIP-chip assays (Promoter Arrays)
It has been proposed that ZNF217, which is amplified at 20q13 in various tumors, plays a key role during neoplastic transformation. ZNF217 has been purified in complexes that contain repressor proteins such as CtBP2, suggesting that it acts as a transcriptional repressor. However, the function of ZN...
ORGANISM(S): Homo sapiens 
2010-06-12 | E-GEOD-6625 | biostudies-arrayexpress
ProteomicsMapping Isoform Abundance and Interactome of the Endogenous TMPRSS2-ERG Fusion Protein by Orthogonal Immunoprecipitation-Mass Spectrometry Assays
Here, we developed immunoprecipitation-mass spectrometry assays for the measurement of a low-abundance T1E4 TMPRSS2-ERG fusion protein, its isoforms and its interactome in VCaP prostate cancer cells.
ORGANISM(S): Homo sapiens (Human) 
2021-03-29 | PXD021236 | Pride
Tmprss2-erg fusion  vcap cells  erg protein isoforms  proteomics  mass spectrometry  selected reaction monitoring  erg interactome  cbaf complex  canonical swi/snf complex 
TranscriptomicsImprovements in sample preparation for ChIP-chip assays
We have improved the new protocol for ChIP-chip by using pooling method. The new method has produced reproducible binding patterns and low background signals. We have performed and compared three methods for ChIP-chip samples: LMPCR, 10 Pooled Samples and WGA.
ORGANISM(S): Homo sapiens 
2010-07-01 | E-GEOD-5251 | biostudies-arrayexpress
UnknownA Microfluidic Device with Integrated Sonication and Immunoprecipitation for Sensitive Epigenetic Assays.
Not available
S-EPMC4741277 | biostudies-literature
UnknownDiagnostic performance of automated plasma amyloid-β assays combined with pre-analytical immunoprecipitation.
Not available
S-EPMC9450259 | biostudies-literature
GenomicsIntegrated analysis based on RNA immunoprecipitation sequencing (RIP-seq) and m5C methylated RNA immunoprecipitation and sequencing (MeRIP-seq) assays to identify the target genes of NSUN2/YBX1-m5C axis in ESCC progression
Integrated analysis based on RNA immunoprecipitation sequencing (RIP-seq) and m5C methylated RNA immunoprecipitation and sequencing (MeRIP-seq) assays to identify the target genes of NSUN2/YBX1-m5C axis in ESCC progression
PRJNA1047076 | ENA
TranscriptomicsCREB ChIP-chip and HaloCHIP-chip experiments
Regulation of gene expression is essential for normal development and cellular growth. Transcriptional events are tightly controlled both spatially and temporally by specific DNA-protein interactions. In this study we finely map the genome-wide targets of the CREB protein across all known and predi...
ORGANISM(S): Homo sapiens 
2010-05-22 | E-GEOD-18347 | biostudies-arrayexpress
GenomicsChromatin immunoprecipitation sequencing of senataxin binding within CIN 612 cells
Chromatin immunoprecipitation sequencing of senataxin binding within CIN 612 cells
PRJEB102620 | ENA
TranscriptomicsA Chromatin Immunoprecipitation Approach Based on BAC Transgenesis
We have devised a technology that enables chromatin immunoprecipitation (ChIP) experiments in cultured human cells by circumventing both the laborious production of protein-specific antibodies and the optimization of crosslinking/immunoprecipitation conditions for each protein. Our approach utilizes...
ORGANISM(S): Homo sapiens 
2010-05-25 | E-GEOD-10845 | biostudies-arrayexpress
TranscriptomicsccpA Chromatin immunoprecipitation on chip of JIR8094 (CD630E) WT strain and a ccpA mutant after 6h of growth in TY
Comparison of ccpA Chromatine immunoprecipitation on chip using 2 strains : JIR8094 (CD630E) wild type strain and a ccpA mutant after 6h of growth in TYG. This experimental procedure was designed to investigate the consensus binding site, CRE, of pleiotropic regulator ccpA. Two-conditions experiment...
ORGANISM(S): [Clostridium] difficile 
2012-11-13 | E-GEOD-34971 | biostudies-arrayexpress
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