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Krüppel-like factor 2 (Klf2) is a DNA-binding transcription factor that regulates embryonic stem cell-specific gene expression. Transcription cofactors such as p300 acetyltransferases and Erk kinases interact with Klf2, providing an additional layer of transcription regulation in embryonic stem cell...
ORGANISM(S): Mus Musculus (mouse) 
We report the usage of ChIP-mass spectrometry in identifying proteins and histone modifications involved in Drosophila dosage compensation. We identified a chromatin targeting factor, CG4747, that is involved in recognition of H3K36me3 and robust recruitment of the Drosophila MSL complex to its corr...
ORGANISM(S): Drosophila melanogaster 
Co-immunoprecipitation of proteins coupled to mass spectrometry is critical for the understanding of protein interaction networks. In instances where a suitable antibody is not available, it is common to graft synthetic tags onto target protein sequences and allowing the use of commercially availabl...
ORGANISM(S): Homo sapiens (Human) 
2023-02-21 | PXD028965 | Pride
The vascular endothelial growth factor A (VEGF) is involved in various physiological processes such as angiogenesis or wound healing but is also crucial in pathological events such as tumor growth. Thus, clinical anti-VEGF treatments have been developed which could already prove to have enormous ben...
ORGANISM(S): Homo sapiens 
RNA-seq and Mass spectrometry analysis reveals the roles of YTHDF1 in aging
Data from ProteomeXchange, PXD ID: PXD002534. File: GR_130129_20_OT3_NT_L3MBTL2_FAB_31_G11_2_HEK293.mzXML. Published as part of Nat Methods. 2015 Jun 29 . From the Abstract: {{i}} ... We describe a mass spectrometry-based standard operating procedure for scoring immunoprecipitation antibody quality...
ORGANISM(S): Homo_sapiens_viruses, Human_female 
Co-immunoprecipitation of proteins coupled to mass spectrometry has transformed modern biology’s understanding of protein interaction networks. These approaches exploit the selective isolation of tagged proteins by affinity enrichment / purification to identify protein binding partners at scale and ...
ORGANISM(S): Homo sapiens (Human) Rabies virus Nishigahara RCEH 
2025-10-15 | PXD026637 | Pride
We performed QKI5 co-immunoprecipitation followed by MS analysis to identify QKI5-interacting proteins in THP-1 cells.
ORGANISM(S): Homo sapiens (Human) 
2021-09-27 | PXD028743 | Pride
Discovery of Klf2 Interactors in Mouse Embryonic Stem Cells by Immunoprecipitation-Mass Spectrometry Utilizing Exogenously Expressed Bait
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