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TranscriptomicsTranscription profiling of Arabidopsis roots and shoots in response to hypoxia using immunopurified mRNA-ribosome complexes - SuperSeries
This SuperSeries is composed of the following subset Series:; GSE14493: Immunopurified mRNA-ribosome complexes expose cell-type specific plasticity during hypoxia in Arabidopsis root tips; GSE14502: Immunopurified mRNA-ribosome complexes expose cell-type specific plasticity in response to hypoxia in...
ORGANISM(S): Arabidopsis thaliana 
2009-10-20 | E-GEOD-14578 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Arabidopsis root tips in response to hypoxia using immunopurified mRNA-ribosome complexes
Plant organs are comprised of distinct cell types with unique assemblages of mRNAs. This is a collection of CEL files of mRNA profiles of the total steady-state mRNAs and polysomal mRNAs of distinct cell types of the root tip of 7-d-old Arabidopsis thaliana seedlings. The cell type specific mRNA pop...
ORGANISM(S): Arabidopsis thaliana 
2009-10-20 | E-GEOD-14493 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of Arabidopsis roots and shoots in response to hypoxia using immunopurified mRNA-ribosome complexes
Plant organs are comprised of distinct cell types with unique assemblages of mRNAs. This is a collection of CEL files of mRNA profiles of the total steady-state mRNAs and polysomal mRNAs of distinct cell types of the whole root and shoot of 7-d-old Arabidopsis thaliana seedlings. The cell type speci...
ORGANISM(S): Arabidopsis thaliana 
2009-10-16 | E-GEOD-14502 | biostudies-arrayexpress
TranscriptomicsIdentification of miR-21 targets in Jurkat cells by AGO2 immunoprecipitation
In order to identify miR-21 targets by a biochemical high-throughput method, we immunopurified RISC Complex and associated mRNAs in both control and miR-21 overexpressing Jurkat cells. Following transduction of miR-21 encoding construct (or a cognate control vector) in Jurkat cells, we used microarr...
ORGANISM(S): Homo sapiens 
2014-10-15 | E-GEOD-37212 | biostudies-arrayexpress
TranscriptomicsImmunopurified mRNA-ribosome complexes expose cell-type specific plasticity during hypoxia in Arabidopsis root tips
Plant organs are comprised of distinct cell types with unique assemblages of mRNAs. This is a collection of CEL files of mRNA profiles of the total steady-state mRNAs and polysomal mRNAs of distinct cell types of the root tip of 7-d-old Arabidopsis thaliana seedlings. The cell type specific mRNA pop...
ORGANISM(S): Arabidopsis thaliana 
2009-10-12 | GSE14493 | GEO
ProteomicsThe Eyes Absent family members EYA4 and EYA1 promote PLK1 activation and successful mitosis through tyrosine dephosphorylation: PLK1 PRM
The aim of these experiments was to quantify PLK1 tyrosine phosphosites with respect to EYA4 and EYA1. PRM was performed on immunopurified PLK1. This was part of a larger project to characterize the effects of EYA4 mediated dephosphorylation on PLK1 functions.
ORGANISM(S): Homo sapiens (Human) 
2024-02-26 | PXD037064 | Pride
Plk1 prm 
ProteomicsIdentification of interacting partners of Ppg1 phosphatase
PP2A-like phosphatase Ppg1 was identified as a regulator of carbon metabolism and enabled adaption to glucose depletion. To elucidate the molecular mechanism, we immunopurified Ppg1 and identified its interacting proteins by mass spectrometry based proteomics.
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-02-29 | PXD049793 | Pride
Glucose signaling Protein-protein interaction Phosphatase Metabolic adaptation 
TranscriptomicsImmunopurified mRNA-ribosome complexes expose cell-type specific plasticity in response to hypoxia in Arabidopsis
Plant organs are comprised of distinct cell types with unique assemblages of mRNAs. This is a collection of CEL files of mRNA profiles of the total steady-state mRNAs and polysomal mRNAs of distinct cell types of the whole root and shoot of 7-d-old Arabidopsis thaliana seedlings. The cell type speci...
ORGANISM(S): Arabidopsis thaliana 
2009-10-12 | GSE14502 | GEO
ProteomicsNIMA-related kinase 9–mediated phosphorylation of the microtubule-associated LC3B protein at Thr-50 suppresses selective autophagy of p62/sequestosome 1
We found that LC3B is phosphorylated in vitro by STK3, STK4, NEK9, and PKCζ. To identify phosphorylation sites following in vitro phosphorylation, GST-LC3B was overexpressed in E. coli, purified using glutathione-Sepharose 4 Fast Flow beads and incubated in kinase assays with recombinant kinases or ...
ORGANISM(S): Homo sapiens (Human) 
2020-01-23 | PXD016681 | Pride
Microtubule-associated lc3b protein Autophagy In vitro phosphorylation 
GenomicsTranscriptional profiling of cells in the dorsal striatum and accumbens nucleus of older Drd1-TRAP (CP73) mice: immunopurified (IP) data
Transcriptional profiling of cells in the dorsal striatum and accumbens nucleus of older Drd1-TRAP (CP73) mice: immunopurified (IP) data
PRJNA595490 | ENA
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