Sort   by:  
 Page size 
ProteomicsTargeted in situ cross-linking for determination of structure and interactions of SARS-CoV-2 proteins in the cellular context
Cross-linking mass spectrometry (XL-MS) has progressed from studying purified protein assemblies in-vitro towards investigating the same assemblies in intact cells, tissues, and even whole organisms (so called “in situ XL-MS”). In situ XL-MS offers the great advantage of reporting on the architectur...
ORGANISM(S): Homo sapiens (Human) 
2021-10-06 | PXD023487 | Pride
Mass-spectrometry Xlms Sars-cov-2 Clms Hek293 Nucleocapsid protein Lc-msms Nsp1 Nsp2 
ProteomicsConventional cell fixation improves performance of in situ crosslinking mass spectrometry while preserving cellular ultrastructure
We developed an approach to in situ XL-MS which utilizes pre-stabilization with formaldehyde prior to crosslinker introduction. Applications of this method can boost crosslinker signal and allows for membrane-impermeable reagents to be used for studying protein-interaction landscapes in intact human...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2024-04-12 | PXD051075 | Pride
In situ xl-ms Formaldehyde fixaiton Crosslinking mass spectrometry 
ProteomicsIn Situ Cross-linking Based Co-Fractionation Mass Spectrometry (XL-CoFrac-MS)
We developed an in situ cross-linking coupled reversed-phase liquid chromatography-based co-fractionation mass spectrometry (XL-CoFrac-MS) platform to analyze drug-induced protein complex changes. A software tool, ChromaQuant, was built to automate chromatographic peak quantification. Using RS4;11 l...
ORGANISM(S): Homo Sapiens 
2025-08-23 | PXD067673 |
ProteomicsOptimized cross-linking mass spectrometry for in situ interaction proteomics
ecent development of mass spectrometer cleavable protein cross-linkers and algorithms for their spectral identification now permits large-scale cross-linking mass spectrometry (XL-MS). Here, we optimized the use of cleavable disuccinimidyl sulfoxide (DSSO) cross-linker for labeling native protein co...
ORGANISM(S): Homo sapiens (Human) 
2019-04-03 | PXD010796 | Pride
Human Cross-linking mass spectrometry Chromatin 
ProteomicsTargeted in situ cross-linking for determination of structure and interactions of SARS-CoV-2 proteins in the cellular context
Discovery of host-pathogen intra-cellular interactions is critical for identification of mechanisms of infection and discovery. Affinity purification based on Mass Spectrometry (AP-MS) approaches can identify the host-pathogen protein-protein interaction network that includes hundreds of cellular pr...
ORGANISM(S): Homo sapiens (Human) 
2021-10-06 | PXD023542 | Pride
Ap-ms Mass-spectrometry Sars-cov-2 Hek293 Nucleocapsid protein Lc-msms Nsp1 Strep-tag Nsp2 
UnknownCell fixation improves performance of in situ crosslinking mass spectrometry while preserving cellular ultrastructure.
Not available
S-EPMC11447256 | biostudies-literature
UnknownOptimized Cross-Linking Mass Spectrometry for in Situ Interaction Proteomics.
Not available
S-EPMC7473601 | biostudies-literature
ProteomicsFacilitating in situ cross-linking and mass spectrometry by antibody-based protein enrichment
Cross-linking of living cells followed by mass spectrometry identification of cross-linked peptides (in situ CLMS) is an emerging technology to study protein structures in their native environment. One of the inherent difficulties of this approach is the high complexity of the samples following cell...
ORGANISM(S): Homo sapiens (Human) 
2022-06-09 | PXD025099 | Pride
Cl-ms Antibody 
UnknownHigher-Order Structural Organization of the Mitochondrial Proteome Charted by In Situ Cross-Linking Mass Spectrometry.
Not available
S-EPMC10651688 | biostudies-literature
MetabolomicsIntegration of time-series meta-omics data reveals how microbial ecosystems respond to disturbance
The development of reliable, mixed-culture biotechnological processes hinges on understanding how microbial ecosystems respond to disturbances. Here we reveal extensive phenotypic plasticity and niche complementarity in oleaginous microbial populations from a biological wastewater treatment plant. W...
2021-04-06 | MTBLS2021 | MetaboLights
Sort   by:  
 Page size