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ProteomicsIdentification of the ubiquitin linkages generated by ANKIB1
moTAP-ANKIB1 was purified from transduced HeLa by using M2 beads followed by streptavidin beads. Once purified, in vitro ubiquitin assay was performed in cell free assay during 1h at 37°C. Purified ANKIB1 was incubated with or without UBE1, UBE2D3, ATP regeneration solution and ubiquitin molecules.
ORGANISM(S): Homo sapiens (Human) 
2026-03-03 | PXD065049 | Pride
In vitro ubiquitin assay E3 ligases 
ProteomicsCRL4(CRBN) ubiquitin ligase profiling
In the 1950s the drug thalidomide administered as a sedative to pregnant women led ot the birth of thousands of children with multiple defects. Despite its teratogenicity, thalidomide and ist IMiD derivatives recently emerged as effective treatments for multiple myeloma and 5q-dysplasia. IMiDs targe...
ORGANISM(S): Homo sapiens 
2014-05-13 | E-GEOD-57554 | biostudies-arrayexpress
TranscriptomicsThe conserved ubiquitin-like protein Hub1 plays a critical role in splicing in human cells
Different from canonical ubiquitin-like proteins, Hub1 does not form covalent conjugates with substrates but binds proteins non-covalently. In Saccharomyces cerevisiae, Hub1 associates with spliceosomes and mediates alternative splicing of SRC1, without affecting pre-mRNA splicing generally. Human H...
ORGANISM(S): Homo sapiens 
2014-05-30 | E-GEOD-56878 | biostudies-arrayexpress
MetabolomicsMetabolome and comparative genome provide insights into secondary metabolites generation of a rare karst-growing Rhododendron in vitro culture

Rhododendron species have the potential to be rich in secondary metabolites with pharmaceutical or industrial value. However, there is a lack of in comprehensive metabolome studies at the genome level, particularly for unique and rare species like R. bailiense which exclusively grows in karst env...

2025-01-02 | MTBLS11581 | MetaboLights
TranscriptomicsExpression Profiles of HepG2 cells treated with genotoxic and non-genotoxic agents
The lack of accurate in vitro assays for predicting in vivo toxicity of chemicals together with new legislations demanding replacement and reduction of animal testing has triggered the development of alternative methods. This study aimed at developing a transcriptomics-based in vitro prediction assa...
ORGANISM(S): Homo sapiens 
2012-08-02 | E-GEOD-28878 | biostudies-arrayexpress
TranscriptomicsThe tumor antigen PRAME is a substrate recognition subunit of a Cul2-based ubiquitin ligase and is associated with active NFY promoters
The human tumor antigen PRAME (Preferentially expressed antigen of melanoma) is frequently overexpressed in tumors. High PRAME levels correlate with poor clinical outcome of several cancers, but the mechanisms by which PRAME could be involved in tumorigenesis remain largely elusive. We applied prote...
ORGANISM(S): Homo sapiens 
2011-07-29 | E-GEOD-26439 | biostudies-arrayexpress
TranscriptomicsMerlin/NF2 Suppresses Tumorigenesis by Inhibiting the E3 Ubiquitin Ligase CRL4DCAF1 in the Nucleus
Current models imply that the FERM domain protein Merlin, encoded by the tumor suppressor NF2, inhibits mitogenic signaling at or near the plasma membrane. Here, we show that the closed, growth inhibitory form of Merlin accumulates in the nucleus, binds to the E3 ubiquitin ligase CRL4DCAF1, and supp...
ORGANISM(S): Mus musculus 
2010-03-09 | E-GEOD-19305 | biostudies-arrayexpress
ProteomicsUbiquitin remenants identification following in vitro assay of 20S and 26S proteasome using naked and ubiquitined cyclin
A systematic and comparative study of the proteolysis products generated by purified human 26S and 20S proteasome following the in vitro cleavage of non-modified (naked), mono-ubiquitinated and poly-ubiquitinated cyclin B.
ORGANISM(S): Homo sapiens (Human) 
2021-11-02 | PXD028912 | Pride
20s Ubiquitin Proteasome 
ProteomicsTwo DOT1 enzymes cooperatively mediate efficient ubiquitin-independent histone H3 lysine 76 tri-methylation in kinetoplastids
In higher eukaryotes, a single DOT1 histone H3 lysine 79 (H3K79) methyltransferase processively produces H3K79me2/me3 from me0 through histone H2B mono-ubiquitin interaction. The early-branched kinetoplastid Trypanosoma brucei harbors the essential H3K76 di-methyltransferase DOT1A and the non-essent...
ORGANISM(S): Trypanosoma Brucei (ncbitaxon:5691) 
2022-11-09 | MSV000090680 | MassIVE
TranscriptomicsscRNA-seq characterisation of in vitro-generated CD8+ exhausted human T cells
scRNA-seq was performed to characterise an in vitro model of T cell exhaustion. CD8+ T cells from two donors underwent chronic stimulation to induce exhaustion and samples were collected from multiple points across the protocol to examine the subsets and transcriptional signatures present.
ORGANISM(S): Homo sapiens 
2026-05-31 | E-MTAB-15435 | biostudies-arrayexpress
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