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Proteomics20S proteasome digestion of HeLa cell extracts
The 20S proteasome is responsible for the catalytic activity of all proteasome complexes. Structural constraints mean that only unfolded, extended polypeptide chains may enter the catalytic core of the 20S proteasome. Here we conducted a comprehensive analysis of the 20S CP substrates in-vitro. We r...
ORGANISM(S): Homo sapiens (Human) 
2018-07-30 | PXD010132 | Pride
20s proteasome digestion Heat soluble proteins 
ProteomicsSystematic identification of 20S proteasome targets
For many years, the ubiquitin-26S proteasome degradation pathway was considered the principal route for proteasomal degradation. However, it is now becoming clear that proteins can also be targeted for degradation by an ubiquitin-independent mechanism mediated by the core 20S proteasome itself. The ...
ORGANISM(S): Homo sapiens (Human) 
2023-12-21 | PXD044399 | Pride
Disorder Degradation 20s proteasome 
ProteomicsUbiquitin remenants identification following in vitro assay of 20S and 26S proteasome using naked and ubiquitined cyclin
A systematic and comparative study of the proteolysis products generated by purified human 26S and 20S proteasome following the in vitro cleavage of non-modified (naked), mono-ubiquitinated and poly-ubiquitinated cyclin B.
ORGANISM(S): Homo sapiens (Human) 
2021-11-02 | PXD028912 | Pride
20s Ubiquitin Proteasome 
ProteomicsStructural Analysis of the 20S Proteasome using Native Mass Spectrometry and Ultraviolet Photodissociation
The yeast 20S proteasome was analyzed using native mass spectrometry combined with UVPD, HCD, and vT-ESI. Nano-LC-MS-EThcD was used to characterize the primary sequence features of the 20S subunits.
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) 
2024-11-01 | PXD056549 | JPOST Repository
ProteomicsSignature activities of 20S proteasome reveal degradation of intact ubiquitin-conjugates in hypoxia
Careful removal of unwanted proteins is necessary for cell survival. The primary constitutive intracellular protease is the 26S proteasome complex, very often found in equilibrium with its catalytic core particle – the 20S subcomplex. Protein degradation by the former is tightly regulated due to pri...
ORGANISM(S): Homo sapiens (Human) 
2021-11-02 | PXD018722 | Pride
26s 20s Peptides Proteasome Hypoxia 
ProteomicsTop-Down Analysis of 20S proteasome
Top-down LC-MS analysis of commercial and in-house immunopurified human 20S proteasome
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-07-10 | MSV000084069 | MassIVE
GenomicsThe constitutive 20S proteasome is required for the maintenance and differentiation of spermatogonia in mice
The constitutive 20S proteasome is required for the maintenance and differentiation of spermatogonia in mice
PRJNA1258215 | ENA
Proteomics20S proteasomes exported by malaria parasite promote its growth
Mature Red Blood Cells (RBCs) lack internal organelles and canonical defense mechanisms, making them both a fascinating host cell, in general, and an intriguing choice for the deadly malaria parasite Plasmodium falciparum (Pf), in particular. While growing inside RBCs, Pf are known to secrete multip...
ORGANISM(S): Homo sapiens (Human) 
2021-01-04 | PXD018154 | Pride
Malaria Phosphorylation Proteasome 
TranscriptomicsGene expression of Ewing sarcoma cell lines treated with 20S proteasome inhibitors and benzyl‐4‐piperidone compounds
We compared the expression changes in Ewing sarcoma cell lines following treatment with 2 known 20S proteasome inhibitors versus 2 novel compounds
ORGANISM(S): Homo sapiens 
2017-04-25 | GSE80640 | GEO
Proteomics20S proteasomes exported by the malaria parasite promote its growth
Mature Red Blood Cells (RBCs) lack internal organelles and canonical defense mechanisms, making them both a fascinating host cell, in general, and an intriguing choice for the deadly malaria parasite Plasmodium falciparum (Pf), in particular. Pf, while growing inside its natural host, the human RBC,...
ORGANISM(S): Plasmodium falciparum (isolate 3D7) 
2021-02-04 | PXD023353 | Pride
Rbcs Malaria Proteasome Evs 
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