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ProteomicsA simplified in vivo cross-linking mass spectrometry strategy for proteome-wide studies.
Chemical cross-linking (XL) coupled to mass spectrometry (MS) has become a powerful approach to probe the structure of protein assemblies. Although most of the applications concerned purified complexes, latest developments focus on large-scale in vivo studies. Pushing in this direction, we describe ...
ORGANISM(S): Neisseria meningitidis Bacteria 
2021-02-25 | PXD021553 | Pride
Click-chemistry Mass spectrometry Structural biology In vivo biochemistry Cross-linking 
ProteomicsCross-linking/mass spectrometry analysis of AUGMIN complex
We applied cross-linking/mass spectrometry to characterize in vivo Augmin from Drosophila in absence of any other structural information. The identified cross-links revealed topology of the Augmin complex and allowed us to predict potential interfaces between Augmin and γ-TuRC.
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2017-04-04 | PXD006246 | Pride
Augmin complex Cross-linking/mass spectromentry 
ProteomicsCross-linking and mass spectrometry analysis of Trypanosoma brucei proteins
We performed in vivo cross-linking of T. brucei bloodstream forms expressing a V5-tagged phosphatidylinositol phosphate 5-phosphatase (PIP5Pase). Then, performed immunoprecipitations with anti-V5 antibodies to identify proteins that cross-links with PIP5Pase or its interacting partners.
ORGANISM(S): Trypanosoma brucei 
2025-11-11 | PXD059635 | Pride
Cross-linking Trypanosomes Nucleus 
ProteomicsA Glycosidic-Bond-Based Mass-Spectrometry-Cleavable Cross-linker Enables In Vivo Cross-linking for Protein Complex Analysis
Chemical cross-linking mass spectrometry (CXMS) has emerged as a powerful technology to analyze protein complex structure and interaction. However, the spectral fragmentation behavior and spectral data retrieval of cross-linked peptides are more complex than single peptides. In this study, we design...
ORGANISM(S): Homo Sapiens Saccharomyces Cerevisiae 
2023-12-06 | PXD047554 |
ProteomicsIn planta chemical crosslinking and mass spectrometry analysis of protein structure and interaction in Arabidopsis
Mass spectrometry analysis in combination with the site-specific chemical cross-linking has emerged as a powerful method in study of three-dimensional structure of protein complex and in mapping of protein-protein interactions (PPIs). Even though in vitro cross-linking experiments have been widely a...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2016-06-28 | PXD003658 | Pride
Arabidopsis Mass spectrometry Disuccinimidyl suberate In planta chemical cross-linking Interactomics Exhaustive cross-linked peptides identification tool 
ProteomicsIn culture cross-linking of bacterial cells reveals large scale dynamic protein-protein interactions at the peptide level.
Identification of dynamic protein-protein interactions at the peptide level on a proteomic scale is a challenging approach that is still in its infancy. We have developed a system to cross-link cells directly in culture with the special lysine cross-linker bis(succinimidyl)-3-azidomethyl-glutarate (...
ORGANISM(S): Bacillus subtilis subsp. subtilis str. 168 
2017-05-23 | PXD006287 | Pride
Escherichia coli Bacillus subtilis Rna polymerase Membrane permeability Transcription. Bamg (bis(succinimidyl)-3-azidomethyl-glutarate) In vivo cross-linking Translation 
ProteomicsDevelopment of in planta chemical cross-linking-based quantitative interactomics in Arabidopsis
An in planta chemical cross-linking-based quantitative interactomics (IPQCX-MS) workflow has been developed to investigate in vivo protein-protein interactions and alteration in protein structures in a model organism, Arabidopsis thaliana. A chemical cross-linker, azide-tag-modified disuccinimidyl p...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-08-14 | PXD008161 | Pride
Arabidopsis thaliana Ecl2 In planta quantitative interactomics Ipqcx-ms In vivo cross-linking 4c proteomic workflow 
ProteomicsNovel roles for ribosomal histidine methylation revealed by proteomics and large-scale cross-linking mass spectrometry
The methylation of histidine residues is increasingly found to be both prevalent throughout the proteome, and also relevant to human disease. Hpm1p mono-methylates H243 in the ribosomal protein Rpl3 and represents the only histidine methyltransferase in Saccharomyces cerevisiae. Interestingly, the h...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-08-12 | PXD027953 | Pride
Cross-linking Yeast Silac Histidine methylation 
ProteomicsQuantitative cross-linking analysis of chemoresistant HeLa cells
Chemoresistance is a common mode of therapy failure for many cancers. Tumors develop resistance to chemotherapeutics through a variety of mechanisms, with proteins serving pivotal roles. Changes in protein conformations and interactions affect the cellular response to environmental conditions cont...
ORGANISM(S): Homo sapiens (Human) 
2015-09-15 | PXD002378 | Pride
Multidrug resistant cancer Chemical cross-linking Mass spectrometry Protein interactions 
ProteomicsIn vivo cross-linking supports a head-to-tail mechanism for regulation of the plant plasma membrane P-type H+-ATPase
In higher plants, a P-type proton pumping ATPase generates the proton-motive force essential for the function of all other transporters and for proper growth and development. X-ray crystallographic studies of the plant plasma membrane proton pump have provided information on amino acids involved in...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-09-24 | PXD010892 | Pride
And arabidopsis thaliana Mass spectrometry Protein cross-linking Protein-protein interaction H+-atpase 
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