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ProteomicsThe SILAC fly allows for accurate protein quantification in vivo
Stable isotope labeling by amino acids in cell culture (SILAC) is widely used to quantify protein abundance in tissue culture cells. Until now, the only multicellular organism completely labeled at the amino acid level was the laboratory mouse. The fruit fly Drosophila melanogaster is one of the mos...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2015-10-12 | PXD003040 | Pride
Silac In vivo Drosophila 
ProteomicsAn Enhanced in vivo SILAC model for Quantification of Drug Metabolism Enzymes
Many of the enzymes involved in xenobiotic metabolism are maintained at a low basal level and are only synthesized in response to activation of upstream sensor/effector proteins. This induction can have implications in a variety of contexts, particularly during the study of the pharmacokinetics, pha...
ORGANISM(S): Mus musculus (Mouse) 
2015-01-09 | PXD001210 | Pride
In vivo silac Pharmacology Drug-drug interaction Xenobiotic metabolism Drug metabolism Toxicology Cytochrome p450 
ProteomicsA Targeted in Vivo SILAC Approach for Quantification of Drug Metabolism Enzymes: Regulation by the Constitutive Androstane Receptor
The modulation of drug metabolism enzyme (DME) expression by therapeutic agents is a central mechanism of drug–drug interaction and should be assessed as early as possible in preclinical drug development. Direct measurement of DME levels is typically achieved by Western blotting, qPCR, or microarray...
ORGANISM(S): Mus musculus (Mouse) 
2014-02-14 | PXD000675 | Pride
Drug metabolism  drug−drug interaction  constitutive androstane receptor  protein quantification  targeted in vivo silac 
UnknownThe SILAC fly allows for accurate protein quantification in vivo.
Not available
S-EPMC2953914 | biostudies-literature
OtherAdvanced identification of proteins in uncharacterized proteomes by pulsed in vivo SILAC
TRANCHE KEY: EfXsK8WKQKnCiGubVrXxCmmygO9Wbb8TYBBFZgdSBwla6M3AjwhqeHpWgNp8DijuGCbKH49+jBc625dSmgLPaNuv0JgAAAAAAAAB0w== Newt Tail Raw Data 12. A pulsed SILAC-based mass spectrometry method was used , which is based on the detection of paired peptides after 13C6-lysine incorporation into proteins in vi...
ORGANISM(S): Stropicalis Ncbi, Xlaevis Ncbi, Caudata 
GPM32300000779 | GPMDB
ProteomicsIn vivo SILAC-based proteomics reveals phosphoproteome changes during mouse skin carcinogenesis
Cancer progresses through distinct stages and mouse models recapitulating traits of this progression are frequently used to explore genetic, morphological and pharmacological aspects of tumor development. To complement genomic investigations of this process, here we quantify phosphoproteomic changes...
ORGANISM(S): Mus musculus (Mouse) 
2013-03-13 | PXD000821 | Pride
Mouse Silac Phosphoproteomics Skin carcinogenesis 
UnknownIdentification of disease specific pathways using in vivo SILAC proteomics in dystrophin deficient mdx mouse.
Not available
S-EPMC3650321 | biostudies-literature
ProteomicsSpatial SILAC Regorafenib Treated Bottom-up Proteomic Analysis
Developing effective model systems to evaluate new potential chemotherapeutic reagents is critical. Three-dimensional cell cultures, such as spheroids, aid in bridging the gap between commonly used monolayer cell cultures and significantly more complex and expensive animal models. Spheroid model sys...
ORGANISM(S): Homo sapiens (Human) 
2024-08-09 | PXD038958 | Pride
Spatial silac Regorafenib Spheroids 
UnknownA targeted in vivo SILAC approach for quantification of drug metabolism enzymes: regulation by the constitutive androstane receptor.
Not available
S-EPMC3923450 | biostudies-literature
ProteomicsCombining data independent acquisition with spike-in SILAC (DIA-SiS) improves proteome coverage and quantification )
Data Independent Acquisition (DIA) is increasingly preferred over Data Dependent Acquisition (DDA) due to its higher throughput and fewer missing values. Whereas DDA often utilizes stable isotope labeling to improve quantification, DIA mostly relies on label-free approaches. Efforts to integrate DIA...
ORGANISM(S): Homo sapiens (Human) Escherichia coli 
2024-10-14 | PXD052080 | Pride
Multiplexing Dia Plexdia Silac Proteomics Benchmark 
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