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ProteomicsUpdated in-gel digestion for protein analysis by mass spectrometry
The in-gel digestion of proteins for analysis by liquid chromatograph mass spectrometry has been used since the early 1990s. Although several improvements have contributed to increasing the quality of the data obtained, many recent publications still use sub-optimal approaches. We present an updated...
ORGANISM(S): Homo sapiens (Human) 
2018-10-02 | PXD009600 | Pride
Proteomics Mass spectrometry In-gel digestion Trypsin 
ProteomicsHit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments.
Traditional in gel-digestion procedures require extensive sample handling, are prone to contamination and not compatible with high-throughput sample preparation. To address these shortcomings, we have modified the conventional in-gel digestion procedure for high-throughput proteomics studies. The mo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-16 | PXD009510 | Pride
Large-scale In-gel digestion Proteomics Tryptic digest Fractionation Native protein complex Sample preparation High-throughput Lc-ms/ms 
ProteomicsHit-Gel: Streamlining in-gel protein digestion for high-throughput proteomics experiments.
Traditional in gel-digestion procedures require extensive sample handling, are prone to contamination and not compatible with high-throughput sample preparation. To address these shortcomings, we have modified the conventional in-gel digestion procedure for high-throughput proteomics studies. The mo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-16 | PXD009485 | Pride
Large-scale In-gel digestion Proteomics Tryptic digest Fractionation Native protein complex Sample preparation High-throughput Lc-ms/ms 
ProteomicsCBB staining showed beneficial effects on proteomic analysis employing PAGE separation followed with whole-gel slicing, in-gel digestion and quantitative LC-MS/MS
Proteomic analysis by combining PAGE separation, gel slicing and slice-by-slice LC-MS/MS has been frequently reported in recent years. Since the MS analysis would provide identities and quantities of the proteins along the whole lane, visualization by dye staining could be skipped to save time and ...
ORGANISM(S): Rattus norvegicus (Rat) 
2019-02-16 | PXD011689 | Pride
Sds-page In-gel digestion Proteomic analysis Cbb r-250 staining Whole gel slicing Quantitative lc-ms/ms Nondenaturing page 
ProteomicsIn-gel tryptic digestion of secondary band(s) in purified FrhA protein preparations
For downstream cryo-EM analyses, we used rapid MALDI-TOF mass profiling to verify the identity of the proteins below the upper FrhA band in our purified preparations.
ORGANISM(S): Escherichia coli 
2026-10-19 | PXD078374 | Pride
Purified frha Maldi-tof Peptide mass fingerprinting In-gel tryptic digestion 
UnknownProteomic analysis of endoscopically (endoscopic pancreatic function test) collected gastroduodenal fluid using in-gel tryptic digestion followed by LC-MS/MS.
Not available
S-EPMC3210639 | biostudies-literature
UnknownUpdates of the In-Gel Digestion Method for Protein Analysis by Mass Spectrometry.
Not available
S-EPMC6492177 | biostudies-literature
ProteomicsIdentifying proteins of mealworms, crickets and chicken breast by LC-MS analysis after tryptic-in gel digestion
Edible mealworms and crickets are high protein food sources, which have recently emerged on the European market. As the production of edible insects is more sustainable than conventional livestock proteins, they are a promising alternative protein source for human consumption. Protein compositions o...
ORGANISM(S): Acheta domesticus Gallus gallus (Chicken) Tenebrio molitor 
2023-06-23 | PXD041521 | Pride
Peptide mass fingerprinting  insect  tenebrio molitor  acheta domesticus  chicken 
ProteomicsQuantitative assessment of the effects of trypsin digestion methods on immunoprecipitation-mass spectrometry-based protein-protein interaction analysis
Immunoprecipitation-mass spectrometry (IP-MS) has become the method of choice for discovering protein-protein interaction (PPI) under native conditions. The success of the IP-MS depends on the efficiency of trypsin digestion and recovery of the tryptic peptides for MS analysis. Several different pro...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) Homo sapiens (Human) 
2017-07-25 | PXD005984 | Pride
Rela interactome Trypsin digestion Protein-protein interaction 
ProteomicsMS/MS analysis of in-gel tryptic peptides of wild-type and mutants of glutamine amidotransferase from Methanocaldococcus jannaschii
A stable post-translational modification, succinimide (SNN), found in Methanocaldococcus jannaschii (Mj) glutamine amidotransferase (GATase) is shown to impart hyper-thermostability to the protein. To explore the role of neighbouring residues in enabling deamidation of Asn109 to SNN, intact protein ...
ORGANISM(S): Methanocaldococcus jannaschii 
2026-06-08 | PXD075155 | Pride
Lc-ms/ms Succinimide Deamidation 
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