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ProteomicsInteraction with IP6K1 supports pyrophosphorylation of substrate proteins by the inositol pyrophosphate 5-IP7
HEK293T cells transiently transfected to express either SFB-tagged GFP or SFB-tagged IP6K1 were lysed in NETN buffer (20 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1 mM EDTA, 0.5% Nonidet P-40) containing protease and phosphatase inhibitor cocktails (Merck), at 4 degree C for 60 min. Cell debris were removed...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-06-20 | MSV000092218 | MassIVE
ProteomicsA GAF domain mediates inositol pyrophosphate substrate channeling in PPIP5K phosphatases
Inositol pyrophosphates are highly phosphorylated nutrient messengers. The final step of their biosynthesis is catalyzed by diphosphoinositol pentakisphosphate kinase (PPIP5K) enzymes, which are conserved among fungi, plants, and animals. PPIP5Ks contain an N-terminal kinase domain that generates th...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2024-10-07 | PXD056020 | Pride
Inositol pyrophosphate Schizosaccharomyces pombe Histidine acid phosphatase Arabidopsis thaliana. hdx-ms Phytase Saccharomyces cerevisiae Enzyme mechanism Phosphate homeostasis Gaf domain Inositol pyrophosphate phosphatase 
TranscriptomicsInositol pyrophosphate dynamics impact fission yeast phosphate homeostasis via modulation of RNA 3' processing and transcription termination
This RNA-Seq analysis compares gene expression of the Inositol pyrophosphate phosphatase mutant aps1∆ and Inositol pyrophsophate kinase mutant asp1-D333A contrasting to the WT in the fission yeast S. pombe
ORGANISM(S): Schizosaccharomyces pombe 
2019-06-16 | GSE131237 | GEO
GenomicsInositol pyrophosphate dynamics impact fission yeast phosphate homeostasis via modulation of RNA 3' processing and transcription termination
Inositol pyrophosphate dynamics impact fission yeast phosphate homeostasis via modulation of RNA 3' processing and transcription termination
PRJNA543029 | ENA
UnknownInositol pyrophosphate catabolism by three families of phosphatases regulates plant growth and development.
Not available
S-EPMC11611267 | biostudies-literature
UnknownAsp1 from Schizosaccharomyces pombe binds a [2Fe-2S](2+) cluster which inhibits inositol pyrophosphate 1-phosphatase activity.
Not available
S-EPMC4641441 | biostudies-literature
UnknownThe InsP7 phosphatase Siw14 regulates inositol pyrophosphate levels to control localization of the general stress response transcription factor Msn2.
Not available
S-EPMC7029108 | biostudies-literature
UnknownActivities, substrate specificity, and genetic interactions of fission yeast Siw14, a cysteinyl-phosphatase-type inositol pyrophosphatase.
Not available
S-EPMC10653929 | biostudies-literature
UnknownA two-way switch for inositol pyrophosphate signaling: Evolutionary history and biological significance of a unique, bifunctional kinase/phosphatase.
Not available
S-EPMC9383039 | biostudies-literature
UnknownTwo bifunctional inositol pyrophosphate kinases/phosphatases control plant phosphate homeostasis.
Not available
S-EPMC6731061 | biostudies-literature
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