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MetabolomicsThe dynamic clustering of insulin receptor underlies its signaling and is disrupted in insulin resistance
Insulin receptor (IR) signaling is central to normal metabolic control and is dysregulated in metabolic diseases such as type 2 diabetes. We report here that IR is incorporated into dynamic clusters at the plasma membrane, in the cytoplasm and in the nucleus of human hepatocytes and adipocytes. Insu...
2022-12-05 | MTBLS6505 | MetaboLights
MetabolomicsGlobal Analyses of Selective Insulin Resistance in Hepatocytes due to Palmitate Lipotoxicity
Obesity is tightly linked to hepatic steatosis and insulin resistance. One feature of this association is the paradox of selective insulin resistance: insulin fails to suppress hepatic gluconeogenesis but activates lipid synthesis in the liver. How lipid accumulation interferes selectively with some...
2018-04-26 | MTBLS582 | MetaboLights
TranscriptomicsExpression data from insulin-treated human primary fibroblasts and effects of U0126 on insulin-induced gene expression
We carried out a high throughput analysis of insulin-induced kinase signaling pathways in primary fibroblasts from 35 unrelated individuals. We found that extensive individual variation exists in induction of various signaling pathways. ERK signaling displayed the greatest variation, which led to ...
ORGANISM(S): Homo sapiens 
2015-08-03 | E-GEOD-21891 | biostudies-arrayexpress
MetabolomicsFFAR4-mediated IL-6 release from islet macrophages promotes insulin secretion and is compromised in type-2 diabetes

The function of islet macrophages is poorly understood. They promote glucose-stimulated insulin secretion (GSIS) in lean mice, however, the underlying mechanism has remained unclear. We show that activation of the free fatty acid receptor FFAR4 on islet macrophages leads to interleukin-6 (IL-6) r...

2026-08-19 | MTBLS12317 | MetaboLights
ProteomicsHigh-throughput phosphoproteomics reveals in vivo insulin signaling dynamics
In this project we report 133 biologically distinct mouse liver cell (Hepa 1-6 and FL83B) and mouse tissue (brain, kidney, and liver) phosphoproteomes prepared using a newly developed scalable, single-run phosphoproteomics platform, using titanium dioxide (TiO2) based phosphopeptide enrichment and p...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2018-06-25 | MSV000082502 | MassIVE
MetabolomicsBrown and beige adipose tissue regulate systemic metabolism through a metabolite interorgan signaling axis
Brown and beige adipose tissue are emerging as distinct endocrine organs. These tissues are functionally associated with skeletal muscle, adipose tissue metabolism and systemic energy expenditure, suggesting an interorgan signaling network. Using metabolomics, we identify 3-methyl-2-oxovaleric acid,...
2022-03-03 | MTBLS2436 | MetaboLights
MetabolomicsInsulin Resistance-Associated Bifidobacterium longum Ameliorates Prediabetes via the 6-ketoLCA/TGR5/GLP-1 axis

Background Prediabetes, a global health concern characterized by insulin resistance (IR) and closely linked to obesity, was investigated in this study to identify key gut microbiota characteristics. The development of insulin resistance is associated with gut microbiota. Therefore, identifying ke...

2026-01-14 | MTBLS13684 | MetaboLights
ProteomicsDefective insulin receptor signaling in hPSCs skews pluripotency and negatively perturbs neural differentiation
Proteomics and phosphoproteomics analyses of two human pluripotent stem cell (hPSC) lines with stable insulin receptor knock down. 6-plex TMT-based strategy was applied to compare control versus insulin receptor (IR) knockdown (n=3 replicates for each condition) for each cell lines (CHB8 and H9). D...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2020-04-16 | MSV000085298 | MassIVE
TranscriptomicsFunction-based discovery of significant transcriptional temporal patterns in insulin-stimulated muscle cells
Background: Insulin's effect on protein synthesis (translation of transcripts) and post-translational modifications, especially those involving reversible modifications such as phosphorylation of various signaling proteins, are extensively studied. On the other hand, insulin's effect on the transcri...
ORGANISM(S): Rattus norvegicus 
2012-05-02 | E-GEOD-28997 | biostudies-arrayexpress
ProteomicsAnalytical Comparison of absolute quantification strategies to investigate the Insulin signaling pathway in fat cells
Mass spectrometry based targeted proteomics is the most sensitive approach to answer and address specific biological questions in an accurate and quantitative fashion. However, the data analysis design used for such quantification varies in the field leading to discrepancies in the reported values. ...
ORGANISM(S): Mus Musculus 
2022-06-13 | PXD026204 | panorama
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